Chemistry Department, Faculty of Mathematics and Natural Science, University of Mataram, Mataram 83125, Indonesia.
Molecules. 2019 Oct 29;24(21):3895. doi: 10.3390/molecules24213895.
N-methyl fatty hydroxamic acid (N-MFHA), which is a derivative of hydroxamic acid (HA), was synthesized from ketapang seed oil ( L.). In general, HAs have wide applications due to their chelating properties and biological activities. N-MFHAs were synthesized using immobilized lipase (Lipozyme TL IM) in biphasic medium which was the ketapang seed oil dissolved in hexane and N-methylhydroxylamine dissolved in water. The products were characterized through color testing and FT-IR spectroscopy after purification. Various factors affecting the enzyme activity investigated in the study included the effect of incubation time, the amount of lipase used, and the temperature. On the basis of the results, the optimum conditions for the synthesis of N-MFHA obtained are 25 h of incubation time, a temperature of 40 °C, and a ratio of 1:100 for the amount of enzyme (g)/oil (g). At the optimum conditions of the reaction, 59.7% of the oils were converted to N-MFHA.
N-甲基脂肪族羟肟酸（N-MFHA）是羟肟酸（HA）的衍生物，由桄榔籽油（L.）合成。一般来说，由于其螯合特性和生物活性，HAs 具有广泛的应用。N-MFHAs 是在两相介质中使用固定化脂肪酶（Lipozyme TL IM）合成的，该两相介质是溶解在己烷中的桄榔籽油和溶解在水中的 N-甲基羟胺。产物经过纯化后通过颜色测试和 FT-IR 光谱进行表征。该研究中研究了影响酶活性的各种因素，包括孵育时间、使用的脂肪酶量和温度的影响。根据结果，获得的 N-MFHA 合成的最佳条件为孵育时间 25 h、温度 40°C 和酶（g）/油（g）的比例为 1:100。在反应的最佳条件下，59.7%的油转化为 N-MFHA。
