Matvienko N I, Troianovskaia I N, Zheleznaia L A, Iarchuk O B
Mol Biol (Mosk). 1985 Nov-Dec;19(6):1457-65.
The deletions in tandem prophage lambda appear with high frequency (to 10%) in rec A- strain of Escherichia coli. The deletions were shown by marker rescue and hybridization of fragments of DNA on nitrocellulose filters with nick-translated phage lambda DNA localized only in prophage area. Right and left att sites are not involved. The majority of defective lysogens had all regulatory regions and deletions of late structural genes. These strains may be used for construction of the host-vector systems with the strongest promoter p'R of phage lambda.
串联噬菌体λ的缺失在大肠杆菌rec A-菌株中高频出现（高达10%）。通过标记拯救以及将硝酸纤维素滤膜上的DNA片段与经切口平移的噬菌体λ DNA杂交，证实这些缺失仅定位于原噬菌体区域。左右att位点未涉及。大多数缺陷溶原菌具有所有调控区域且晚期结构基因缺失。这些菌株可用于构建具有噬菌体λ最强启动子p'R的宿主-载体系统。
