Hernandez V J, Edlind T D, Young R F, Ihler G M
Gene. 1985;33(3):363-5. doi: 10.1016/0378-1119(85)90245-8.
Near the right end of phage lambda DNA, between gene Rz and the cos site, are 2050 bp of apparently non-coding DNA. We have cloned a lambda DNA fragment containing this DNA into a plasmid and constructed a deletion, omega l, extending from a site within the Rz gene to a site about 560 bp from cos. This deletion could be recombined into viable lambda phage at a frequency equal to that observed for the undeleted sequence. Recombinant phage lambda carrying the omega l deletion were demonstrated to have the same burst size and kinetics of phage production as undeleted lambda. The omega l deletion can be used to extend the capacity of lambda cloning vectors and to provide a region for the insertion of heterologous DNA which should exhibit controllable high level expression from the lambda late promoter, p'R.
在噬菌体λ DNA 的右端附近，基因 Rz 和粘性末端位点（cos 位点）之间，有一段 2050 碱基对的明显非编码 DNA。我们已将包含这段 DNA 的λ DNA 片段克隆到一个质粒中，并构建了一个缺失片段ωl，它从 Rz 基因内的一个位点延伸至距 cos 约 560 碱基对的一个位点。这个缺失片段能够以与未缺失序列相同的频率重组到有活力的λ噬菌体中。携带ωl 缺失的重组噬菌体λ被证明具有与未缺失的λ相同的裂解量和噬菌体产生动力学。ωl 缺失可用于扩展λ克隆载体的容量，并提供一个用于插入异源 DNA 的区域，该区域应能从λ晚期启动子 p'R 实现可控的高水平表达。
