内脏利什曼病犬外周血单核细胞和脾脏中的细胞凋亡及一氧化氮生成
Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis.
作者信息
de Martini Cleber Costa, de Andrade Jéssica Thomé, de Almeida Stéfani Karin Martiniano, Oliveira Silva Kathlenn Liezbeth, de Rezende Eugenio Flavia, Dos Santos Paulo Sergio Patto, de Lima Valéria Marçal Felix
机构信息
Department of Clinical Care, Surgery and Animal Reproduction, Laboratory of Cellular Immunology, Faculty of Veterinary Medicine of Araçatuba, Universidade Estadual Paulista, "Julio de Mesquita Filho", FMVA/UNESP, Rua Clovis Pestana, 793, Araçatuba, São Paulo, CEP 16050-680, Brazil.
Department of Clinical Care, Surgery and Animal Reproduction, Laboratory of Cellular Immunology, Faculty of Veterinary Medicine of Araçatuba, Universidade Estadual Paulista, "Julio de Mesquita Filho", FMVA/UNESP, Rua Clovis Pestana, 793, Araçatuba, São Paulo, CEP 16050-680, Brazil.
出版信息
Comp Immunol Microbiol Infect Dis. 2018 Apr;57:1-7. doi: 10.1016/j.cimid.2018.01.003. Epub 2018 Jan 31.
Nitric oxide (NO) is involved in the death of the Leishmania parasite and regulation of apoptosis. We quantified the frequency of cells producing NO and its levels in the peripheral blood mononuclear cells (PBMC), leukocytes from spleen in Visceral Leishmaniasis (VL) symptomatic dogs and correlated NO levels with apoptosis and parasite load in the spleen. The percentage of NO+ cells and CD14+/NO+ was higher in PBMC and spleen cells in infected dogs than in controls. The levels of NO+ and CD14+/NO+ cells was higher in PBMC, but lower spleen of dogs infected than compared to control. Late apoptosis rates increased in PBMC and spleen of infected dogs compared to controls, and the NO levels and apoptosis not showed correlation. There was a positive correlation between the percentage of cells producing NO in the spleen and parasite load. The NO participates in the immune response in the canine VL, but it is not apoptosis inducer.
一氧化氮（NO）参与利什曼原虫寄生虫的死亡及细胞凋亡的调节。我们对内脏利什曼病（VL）有症状犬外周血单个核细胞（PBMC）、脾脏白细胞中产生NO的细胞频率及其水平进行了量化，并将NO水平与脾脏中的细胞凋亡及寄生虫负荷相关联。感染犬的PBMC和脾脏细胞中NO⁺细胞及CD14⁺/NO⁺的百分比高于对照组。与对照组相比，感染犬的PBMC中NO⁺和CD14⁺/NO⁺细胞水平较高，但脾脏中的较低。与对照组相比，感染犬的PBMC和脾脏中晚期凋亡率增加，且NO水平与细胞凋亡无相关性。脾脏中产生NO的细胞百分比与寄生虫负荷呈正相关。NO参与犬类VL的免疫反应，但它不是细胞凋亡诱导剂。
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