[评估3种用于检测抗淋巴细胞脉络丛脑膜炎病毒（LAV）抗体的免疫酶试剂盒。与确证试验的比较。国家输血协会研究小组]

[Evaluation of 3 immuno-enzyme kits for the detection of anti-LAV antibodies. Comparison with confirmation tests. Study Subgroup of the National Blood Transfusion Society].

出版信息

Rev Fr Transfus Immunohematol. 1985 Sep;28(4):325-44.

Abstract

Three enzymo immuno assay kits for the detection of anti-LAV/HTLV III antibodies were evaluated: Diagnostics Pasteur (Elavia) Abbott Laboratories (Abbott/HTLV III) and Organon (Vironostika). A total of 6017 blood donors (5980 unselected and 37 prisoners) and a panel of 55 sero negative or sero positive samples were tested. Very weak differences were noted between these 3 kits: Abbott-HTLV III seems the least specific and the most sensitive and Vironostika the most specific and the least sensitive. ELISA quantitative results are given and compared to the results obtained by indirect immunofluorescence, Western-Blot and radio immuno precipitation.

摘要

对三种用于检测抗LAV/HTLV III抗体的酶免疫分析试剂盒进行了评估：巴斯德诊断公司（Elavia）、雅培实验室（Abbott/HTLV III）和欧加农公司（Vironostika）。共检测了6017名献血者（5980名未经筛选者和37名囚犯）以及一组55份血清阴性或血清阳性样本。这三种试剂盒之间存在非常细微的差异：Abbott-HTLV III似乎特异性最低但敏感性最高，而Vironostika特异性最高但敏感性最低。给出了ELISA定量结果，并与间接免疫荧光、免疫印迹和放射免疫沉淀法获得的结果进行了比较。