Petkova K, Mitov B, Kharalambiev Kh E
Vet Med Nauki. 1985;22(9):27-31.
Attempts were made to culture strains of the bovine pestivirus in organ cultures of tracheal rings. Contrary to other scientists the authors used roller organ cultures of calf tracheal rings. The development of the virus in the cultures was judged by its presence in the maintaining medium through ELISA or by titration in cell cultures of fetal calf kidney. It was found that the two strains tested could be replicated in organ cultures of calf trachea, the intensity of multiplication being strongest between the third and the sixth day as demonstrated by ELISA. It was also established that the various batches of tracheal tissue had varying susceptibility.
人们尝试在气管环的器官培养物中培养牛瘟病毒株。与其他科学家不同，作者使用了小牛气管环的旋转器官培养物。通过ELISA检测维持培养基中病毒的存在或通过在胎牛肾细胞培养物中滴定来判断病毒在培养物中的生长情况。结果发现，所测试的两种毒株能够在小牛气管的器官培养物中复制，ELISA结果表明，在第三天到第六天之间增殖强度最强。还确定了不同批次的气管组织具有不同的敏感性。
