Cholakova R
Vet Med Nauki. 1985;22(9):32-8.
The effectiveness of immunity was studied following a mixed vaccination with live vaccines against infectious bronchitis (strains H120 and H52), Newcastle disease (strain La Sota), and infectious bursitis (strain Th75Vn82). The three vaccines were applied simultaneously via the drinking water, through the spray method, and nasally. Experiments were carried out with a total of 31,466 birds: broilers, growing layers, broiler parents--all without preliminary treatment with biopreparations. Immunity against Newcastle disease was followed up through the hemagglutination-inhibition test and challenging with a virulent virus; against infectious bursitis--through immunodiffusion in agar gel after Ouchterlony; and against infectious bronchitis--through virus-neutralization with strain Beaudette. The birds were treated with mixed vaccines in the following combinations: infectious bronchitis--Newcastle disease; infectious bursitis--Gumboro; infectious bronchitis, Newcastle disease, Gumboro. The simultaneous application of live vaccines against infectious bronchitis, Newcastle disease, and infectious bursitis was shown to be well tolerated with no harmful aftereffects whatever. The immunity built up with the simultaneous use of the three vaccines was not inferior in effectiveness to that conferred with the use of two vaccines or only one of them.
研究了使用针对传染性支气管炎（H120和H52株）、新城疫（La Sota株）和传染性法氏囊炎（Th75Vn82株）的活疫苗进行混合接种后的免疫效果。这三种疫苗通过饮水、喷雾法和滴鼻法同时接种。总共对31466只鸡进行了实验，包括肉鸡、育成蛋鸡、肉种鸡，所有鸡均未预先使用生物制剂进行处理。通过血凝抑制试验和用强毒病毒攻毒来监测新城疫的免疫情况；通过Ouchterlony琼脂凝胶免疫扩散法监测传染性法氏囊炎的免疫情况；通过用Beaudette株进行病毒中和试验监测传染性支气管炎的免疫情况。用以下组合的混合疫苗对鸡进行处理：传染性支气管炎-新城疫；传染性法氏囊炎-甘博罗病；传染性支气管炎、新城疫、甘博罗病。结果表明，同时接种针对传染性支气管炎、新城疫和传染性法氏囊炎的活疫苗耐受性良好，没有任何有害的后遗症。同时使用这三种疫苗产生的免疫力在效果上并不低于使用两种疫苗或仅使用其中一种疫苗所产生的免疫力。
