Li Hailing, Zhao Changlei, Xu Min, Yin Min
Departments of Rehabilitation Medicine.
Emergency, Yidu Central Hospital, Weifang.
Neuroreport. 2018 Oct 17;29(15):1249-1255. doi: 10.1097/WNR.0000000000001090.
Mitogen-activated and stress-activated protein kinase 1 (MSK1), which belongs to the subfamily of MAPK-activated protein kinase, plays an important role in cell proliferation and neoplastic transformation. It has been recently reported that MSK1 overexpression was closely related to the progression of some tumors such as colorectal cancer. However, the clinical significance of MSK1 in glioma has not been addressed. To investigate the potential role of MSK1 in glioma, we first examined the expression pattern of MSK1 in glioma tissues and normal brain tissues using quantitative RT-PCR, and the results showing that MSK1 expression was significantly elevated in glioma tissues compared with normal brain tissues. The clinical relevance of MSK1 expression level was then analyzed, and we found that high expression of MSK1 was closely related to the larger tumor size and advanced WHO grade. Univariate and multivariate analyses revealed that glioma patients with higher expression of MSK1 had poorer overall survival, and MSK1 was identified as an independent unfavorable prognosis factor. In addition, the effects of MSK1 on glioma cells were tested through cellular experiments, and we demonstrated that MSK1 can promote proliferation and invasion capacities of tumor cells. In conclusion, patients with glioma with higher MSK1 expression were more predisposed to poorer clinical outcomes and unfavorable prognosis, indicating the potential role of MSK1 as a novel clinical biomarker and therapeutic target.
丝裂原活化蛋白激酶1（MSK1）属于丝裂原活化蛋白激酶激活的蛋白激酶亚家族，在细胞增殖和肿瘤转化中起重要作用。最近有报道称，MSK1的过表达与某些肿瘤（如结直肠癌）的进展密切相关。然而，MSK1在胶质瘤中的临床意义尚未得到研究。为了探讨MSK1在胶质瘤中的潜在作用，我们首先采用定量逆转录聚合酶链反应检测了胶质瘤组织和正常脑组织中MSK1的表达模式，结果显示与正常脑组织相比，MSK1在胶质瘤组织中的表达显著升高。随后分析了MSK1表达水平的临床相关性，我们发现MSK1的高表达与肿瘤体积较大和世界卫生组织（WHO）分级较高密切相关。单因素和多因素分析显示，MSK1表达较高的胶质瘤患者总生存期较差，且MSK1被确定为独立的不良预后因素。此外，通过细胞实验检测了MSK1对胶质瘤细胞的影响，我们证明MSK1可促进肿瘤细胞的增殖和侵袭能力。总之，MSK1表达较高的胶质瘤患者更容易出现较差的临床结局和不良预后，这表明MSK1作为一种新的临床生物标志物和治疗靶点具有潜在作用。
