Department of Agronomy, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou, 310058, China.
Department of Agronomy, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou, 310058, China.
Plant Physiol Biochem. 2018 Sep;130:248-257. doi: 10.1016/j.plaphy.2018.07.013. Epub 2018 Jul 17.
Barley (Hordeum vulgare) and rice (Oryza sativa) differ greatly in their salt tolerance, although both species belong to the Poaceae family. To understand the mechanisms in the difference of salt tolerance between the two species, the responses of ionome, metabolome and gene expression of Na and K transporters to the different salt treatments were analyzed using 4 barley and 4 rice genotypes differing in salt tolerance. In comparison with 4 rice genotypes, four barley genotypes showed better plant growth, lower shoot Na concentration and higher K concentration at the 9 day after salt treatments. There was a dramatic difference in absolute expression levels of SOS, HKT and NHX family genes between barley and rice, which might account for their difference in Na/K homeostasis and salt tolerance. Moreover, rice leaves accumulated excess Na under salt treatments, which caused serious damages to physiological metabolisms based on metabolomic analysis, but barley leaves had lower Na concentration and small changes in the most metabolites. These results provide useful insights into the molecular mechanism in the difference of salt tolerance between rice and barley.
大麦（Hordeum vulgare）和水稻（Oryza sativa）在耐盐性方面差异很大，尽管它们都属于禾本科。为了了解这两个物种耐盐性差异的机制，我们使用 4 个大麦和 4 个耐盐性不同的水稻基因型，分析了离子组、代谢组和 Na 和 K 转运蛋白基因表达对不同盐处理的响应。与 4 个水稻基因型相比，4 个大麦基因型在盐处理后 9 天表现出更好的生长、较低的茎 Na 浓度和较高的 K 浓度。在 SOS、HKT 和 NHX 家族基因的绝对表达水平上，大麦和水稻之间存在显著差异，这可能是它们在 Na/K 稳态和耐盐性方面存在差异的原因。此外，基于代谢组学分析，水稻叶片在盐处理下积累了过量的 Na，这对生理代谢造成了严重损害，而大麦叶片的 Na 浓度较低，大多数代谢物变化较小。这些结果为水稻和大麦耐盐性差异的分子机制提供了有用的见解。
