Mougin C, Kehrer P, Gaillard R
Ann Endocrinol (Paris). 1985;46(1):61-3.
This study was carried out to evaluate the role of the calcium-calmodulin system in cellular mediation of somatocrinin action by cells producing growth hormone. We compared GH release to intra- and extracellular cAMP levels, after incubation of pituitary cells in monolayer culture with somatocrinin alone or somatocrinin associated with exogenous calmodulin, or specific calmodulin inhibitor (W13). Calmodulin (10(-6) M) decreases somatocrinin - induced cAMP levels, maintaining elevated GH levels. W13 (10(-5) M) increases somatocrinin - induced cAMP and GH levels. Thus GH release induced by somatocrinin is modulated by two interrelated systems, adenylcyclase - cAMP system and Ca++-calmodulin system.
本研究旨在评估钙-钙调蛋白系统在生长激素分泌细胞介导生长激素释放激素作用中的作用。我们将单层培养的垂体细胞分别单独与生长激素释放激素孵育，或与外源性钙调蛋白或特异性钙调蛋白抑制剂（W13）联合生长激素释放激素孵育后，比较生长激素释放与细胞内和细胞外环磷酸腺苷（cAMP）水平。钙调蛋白（10⁻⁶ M）可降低生长激素释放激素诱导的cAMP水平，同时维持生长激素水平升高。W13（10⁻⁵ M）可增加生长激素释放激素诱导的cAMP和生长激素水平。因此，生长激素释放激素诱导的生长激素释放受两个相互关联的系统调节，即腺苷酸环化酶-cAMP系统和Ca²⁺-钙调蛋白系统。
