Mougin C, Brazeau P, Ling N, Lewin M, Guillemin R
C R Acad Sci III. 1984;299(4):83-8.
Growth hormone (GH) release from Rat pituitary cell monolayers in response to synthetic somatocrinin (7.8 to 1,000 pmol/l) is paralleled with an increase in cellular cyclic AMP (cAMP) content and efflux of cAMP in the extracellular medium. Somatostatin and blockers of calcium-dependent cellular mechanisms inhibit somatocrinin-induced GH release but only partially decrease (somatostatin, CoCl2) or even increase (trifluoperazine) cAMP levels. Thus, calcium is required for somatocrinin action and GH release is not simply dependent on stimulation of cAMP metabolism.
大鼠垂体细胞单层对合成促生长激素释放素（7.8至1000皮摩尔/升）的生长激素（GH）释放与细胞内环磷酸腺苷（cAMP）含量的增加以及细胞外培养基中cAMP的流出平行。生长抑素和钙依赖性细胞机制的阻滞剂抑制促生长激素释放素诱导的GH释放，但仅部分降低（生长抑素、氯化钴）或甚至增加（三氟拉嗪）cAMP水平。因此，钙是促生长激素释放素作用所必需的，且GH释放并不简单地依赖于cAMP代谢的刺激。
