Yao Jiwei, Wang Yuan, Dai Yifan, Liu Chung Chiun
Department of Chemical & Biomolecular Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106, United States.
ACS Omega. 2018 Jun 30;3(6):6411-6418. doi: 10.1021/acsomega.8b00634. Epub 2018 Jun 14.
Prostate cancer is prevalent among cancers in men. A simple method for screening of reliable biomarkers is pivotal for early detection of prostate cancer. Prostate-specific antigen (PSA) has been a commonly used biomarker for prostate cancer, in spite of its false-positive limitation. On the other hand, alpha-methylacyl-CoA racemase (AMACR), a metabolic enzyme, has been proven to be a highly expressed biomarker in prostate cancer cells. Therefore, a method or tool, which can detect either PSA or AMACR or both simply, cost effectively, and with high sensitivity and selectivity is desirable. We describe a novel bioconjugated, single-use biosensor capable of detecting both PSA and AMACR antigens in undiluted human serum. The preparation of the biosensor by the bioconjugation mechanism occurred within a day, which could be completed prior to actual testing. The effectiveness of the bioconjugation mechanism and the coverage of the electrode surface of the biosensor were experimentally assessed. Measurements of PSA and AMACR antigens and the specificity of the biosensor were carried out using differential pulse voltammetry. This biosensor was single-use and cost-effective and required a small quantity of test medium and relatively short preparation time, providing a very attractive biosensor for the detection of the biomarkers of prostate cancer.
前列腺癌在男性癌症中很常见。一种筛选可靠生物标志物的简单方法对于前列腺癌的早期检测至关重要。前列腺特异性抗原（PSA）一直是前列腺癌常用的生物标志物，尽管存在假阳性局限性。另一方面，α-甲基酰基辅酶A消旋酶（AMACR）作为一种代谢酶，已被证明是前列腺癌细胞中高表达的生物标志物。因此，需要一种能够简单、经济高效且高灵敏度和高选择性地检测PSA或AMACR或两者的方法或工具。我们描述了一种新型的生物共轭一次性生物传感器，能够检测未稀释人血清中的PSA和AMACR抗原。通过生物共轭机制制备生物传感器在一天内即可完成，可在实际测试前完成。通过实验评估了生物共轭机制的有效性和生物传感器电极表面的覆盖率。使用差分脉冲伏安法对PSA和AMACR抗原进行测量以及评估生物传感器的特异性。这种生物传感器是一次性的且具有成本效益，需要少量测试介质和相对较短的制备时间，为检测前列腺癌生物标志物提供了一种非常有吸引力的生物传感器。
