一种基于PCR的快速方法，可根据ctaC基因序列从密切相关的葡萄球菌物种中鉴别出溶酪巨球菌和犬巨球菌。
A rapid PCR-based method to discriminate Macrococcus caseolyticus and Macrococcus canis from closely-related Staphylococcus species based on the ctaC gene sequence.
作者信息
Mazhar Shahneela, Hill Colin, McAuliffe Olivia
机构信息
Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland; School of Microbiology, University College Cork, Ireland.
School of Microbiology, University College Cork, Ireland; APC Microbiome Institute, Cork, Ireland.
出版信息
J Microbiol Methods. 2018 Sep;152:36-38. doi: 10.1016/j.mimet.2018.07.008. Epub 2018 Jul 17.
Our method exploits the amplification of the cytochrome c oxidase subunit II (ctaC) gene for the screening of Macrococcus caseolyticus and Macrococcus canis in complex microbial communities, and discriminating these species from strains of their sister genus Staphylococcus. Thirteen novel strains of these species were isolated using this approach.
我们的方法利用细胞色素c氧化酶亚基II（ctaC）基因的扩增来筛选复杂微生物群落中的解酪巨球菌和犬巨球菌，并将这些物种与它们的姐妹属葡萄球菌属的菌株区分开来。使用这种方法分离出了这13种新菌株。
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