Laboratory for Characterization & Processing of Polymers, Faculty of Mechanical Engineering, University of Maribor, Smetanova 17, SI-2000 Maribor, Slovenia.
Institute for Chemistry & Technology of Materials, Graz University of Technology, Stremayrgasse 9, AT-8010 Graz, Austria.
Nanomedicine (Lond). 2018 Jul;13(13):1583-1594. doi: 10.2217/nnm-2018-0014.
A novel electrospun biocompatible nanofibrous material loaded with commensal bacteria for potential preventive treatment of the diabetic foot was developed.
MATERIALS & METHODS: Two biocompatible polymers (carboxymethylcellulose and polyethylene oxide) were combined with a bacterium isolate from the skin located between the toes of a healthy adult (identified using a matrix-assisted laser desorption/ionization mass spectrometry-based method as a strain of Staphylococcus epidermidis). Higher bacteria loads in the material were assured through their encapsulation in polyethylenimine. The nanofibrous material was characterized using scanning electron microscopy, zeta-potential measurements and through evaluation of cell growth and viability.
RESULTS & DISCUSSION: nanometer formation was confirmed using scanning electron microscopy, while the zeta-potential measurements revealed successful bacteria encapsulation. Viable and sufficiently growing cells were confirmed prior and after their incorporation.
The prepared materials were proven suitable to deliver viable commensal bacteria in a comparable share to the Staphylococcaceae in the foot microbiome making this approach promising for preventive diabetic foot treatment.
开发了一种新型的电纺生物相容性纳米纤维材料，负载共生菌，用于潜在的糖尿病足预防治疗。
将两种生物相容性聚合物（羧甲基纤维素和聚氧化乙烯）与一种从健康成人脚趾间皮肤分离的细菌（通过基质辅助激光解吸/电离质谱法鉴定为表皮葡萄球菌菌株）结合。通过将细菌封装在聚乙烯亚胺中来确保材料中更高的细菌负载。使用扫描电子显微镜、ζ-电位测量以及细胞生长和活力评估对纳米纤维材料进行了表征。
扫描电子显微镜证实了纳米形成，而 ζ-电位测量则表明成功地对细菌进行了封装。在将细胞掺入之前和之后，均确认了存活和充分生长的细胞。
所制备的材料适合输送活的共生菌，其在足部微生物组中的份额与葡萄球菌科相当，这使得该方法有望用于预防糖尿病足的治疗。
