International Research Centre in Critical Raw Materials-ICCRAM, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain.
Department of Chemistry, University of Burgos, Plaza Misael Bañuelos s/n., 09001 Burgos, Spain.
Int J Mol Sci. 2019 Dec 27;21(1):205. doi: 10.3390/ijms21010205.
The ability of commercial monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) to interact with different unicellular systems and biomolecules was studied by analyzing the response of human alveolar carcinoma epithelial cells, the yeast and the bacteria to the presence of different nanoparticle concentrations, and by studying the binding affinity of different microbial enzymes, like the α-l-rhamnosidase enzyme RhaB1 from the bacteria and the AbG β-d-glucosidase from sp. (strain ATCC 21400). An analysis of cytotoxicity on human epithelial cell line A549, (colony forming units, ROS induction, genotoxicity) and (luminescence inhibition) cells determined the potential of both nanoparticle types to damage the selected unicellular systems. Also, the protein binding affinity of the graphene derivatives at different oxidation levels was analyzed. The reported results highlight the variability that can exist in terms of toxicological potential and binding affinity depending on the target organism or protein and the selected nanomaterial.
研究了商用单层氧化石墨烯（GO）和氧化石墨烯纳米胶体（GOC）与不同单细胞系统和生物分子相互作用的能力，方法是分析人肺泡癌细胞、酵母 和细菌 对不同纳米颗粒浓度存在的反应，并研究不同微生物酶的结合亲和力，如细菌 中的α-l-鼠李糖苷酶 RhaB1 和 sp.（ATCC 21400 株）中的 AbG β-d-葡萄糖苷酶。对人上皮细胞系 A549 的细胞毒性分析（集落形成单位、ROS 诱导、遗传毒性）和 （发光抑制）细胞确定了这两种纳米颗粒类型对所选单细胞系统造成损害的潜力。此外，还分析了不同氧化水平的石墨烯衍生物的蛋白质结合亲和力。报告的结果强调了根据目标生物体或蛋白质和所选纳米材料的不同，毒理学潜力和结合亲和力可能存在的可变性。
