Hartmann T, Seuwen K, Adam G
Exp Cell Res. 1986 Mar;163(1):279-86. doi: 10.1016/0014-4827(86)90582-3.
Cellular compartmentation of Ca has been investigated by kinetic analysis of 45Ca efflux from preloaded cells at various states of cell density-dependent proliferation of normal (3T3) and transformed (3T6 and SV40-3T3) mouse cells. Three pools of exchangeable calcium were separated on the basis of their differing exchange kinetics. For each of the cell lines tested, all three compartments decrease with cell density. Significant differences between normal and transformed cells are observed upon quiescence of the normal cells, where the slowly exchanging compartment of normal cells gradually increases, whereas that of the transformed cells continues to decrease (with increasing cell density). Free cytoplasmic Ca2+ concentration as determined by the Quin 2 method, was found to be significantly higher in transformed cells than in normal cells. These results indicate significant differences in Ca homeostasis between normal and transformed cells.
通过对正常（3T3）和转化（3T6及SV40 - 3T3）小鼠细胞在细胞密度依赖性增殖的不同状态下预加载细胞的45Ca流出进行动力学分析，研究了钙的细胞区室化。根据其不同的交换动力学分离出三个可交换钙池。对于所测试的每个细胞系，所有三个区室都随细胞密度降低。当正常细胞静止时，观察到正常细胞和转化细胞之间存在显著差异，此时正常细胞中缓慢交换的区室逐渐增加，而转化细胞的该区室继续降低（随着细胞密度增加）。通过Quin 2方法测定，发现转化细胞中的游离细胞质Ca2 +浓度显著高于正常细胞。这些结果表明正常细胞和转化细胞之间在钙稳态方面存在显著差异。
