Seuwen K, Adam G
Biochem Biophys Res Commun. 1984 Nov 30;125(1):337-45. doi: 10.1016/s0006-291x(84)80373-3.
Total cellular calcium content (determined by atomic absorption spectrometry) of Rat-1 cells transformed by temperature-sensitive Rous sarcoma virus decreases with cell density, but is found not significantly different at permissive and at non-permissive temperature. Kinetic analysis of 45Ca efflux from preloaded cells exhibits three separable pools of exchangeable calcium. The ratio of pool size of the fast-exchanging Ca-compartment (bound to cell surface) to pool size of the intermediate Ca-compartment (cytoplasmic) was found to decrease from 2.5 to 1.3 upon shift from non-permissive to permissive temperature. The slowly exchanging Ca-pool (presumably mitochondrial) did not change significantly upon temperature shift. These and further data demonstrate a close correlation between distribution of cellular Ca among different cellular compartments and characteristics of cellular proliferation, both attributable to the function(s) of a single oncogene.
用原子吸收光谱法测定的、由温度敏感型劳氏肉瘤病毒转化的大鼠1细胞的总细胞钙含量随细胞密度降低，但在允许温度和非允许温度下无显著差异。对预加载细胞的45Ca流出进行动力学分析，发现可交换钙有三个可分离的池。从非允许温度转变为允许温度时，快速交换钙区室（与细胞表面结合）的池大小与中间钙区室（细胞质）的池大小之比从2.5降至1.3。缓慢交换钙池（可能是线粒体）在温度转变时无显著变化。这些以及更多数据表明，细胞内不同区室间钙的分布与细胞增殖特性密切相关，二者均归因于单个癌基因的功能。
