Falkenburg J H, Koning F, Duinkerken N, Fibbe W E, Voogt P J, Jansen J
Exp Hematol. 1986 Feb;14(2):90-6.
The expression of transferrin receptor-associated antigens and of CD11 and CDw15 antigens was investigated on myeloid committed progenitor cells (CFU-GM day 10, CFU-GM day 7, and cluster-forming cells [CFC] day 4), on erythroid committed progenitor cells (BFU-E and CFU-E), and on multilineage progenitor cells (CFU-GEMM). Both complement-dependent cytotoxicity and fluorescence-activated cell-sorting assays were performed. Complement-dependent cytotoxicity appeared to be the more sensitive assay. Transferrin receptor-associated antigens appeared to be clearly present on all myeloid and erythroid committed progenitor cells, but were found to be only weakly expressed on CFU-GEMM. CD11 antigens appeared to be strongly expressed only on mature granulocytes, monocytes, and certain lymphocytes, but not significantly on myeloid committed precursor cells. Surprisingly, CD11 antigens were weakly, but significantly, present on CFU-E. CDw15 antigens appeared to be restricted to myeloid differentiation and were increasingly expressed from CFU-GM day 10 to CFC day 4. Thus, antitransferrin receptor, CD11, and CDw15 antibodies can be used to separate hematopoietic progenitor cells and may be useful tools in the study of hematopoietic differentiation.
研究了转铁蛋白受体相关抗原以及CD11和CDw15抗原在髓系定向祖细胞（第10天的CFU - GM、第7天的CFU - GM和第4天的集落形成细胞[CFC]）、红系定向祖细胞（BFU - E和CFU - E）以及多系祖细胞（CFU - GEMM）上的表达情况。同时进行了补体依赖细胞毒性试验和荧光激活细胞分选试验。补体依赖细胞毒性试验似乎是更敏感的检测方法。转铁蛋白受体相关抗原在所有髓系和红系定向祖细胞上似乎均明显存在，但在CFU - GEMM上仅微弱表达。CD11抗原似乎仅在成熟粒细胞、单核细胞和某些淋巴细胞上强烈表达，而在髓系定向前体细胞上无明显表达。令人惊讶的是，CD11抗原在CFU - E上有微弱但显著的表达。CDw15抗原似乎局限于髓系分化，并且从第10天的CFU - GM到第4天的CFC表达逐渐增加。因此，抗转铁蛋白受体、CD11和CDw15抗体可用于分离造血祖细胞，并且可能是研究造血分化的有用工具。
