Peschel C, Konwalinka G, Geissler D, Majdic O, Stockinger H, Braunsteiner H, Knapp W
Exp Hematol. 1985 Dec;13(11):1211-6.
In the present study we evaluated the reactivity of monoclonal cytotoxic antibodies directed against myeloid differentiation antigens with hemopoietic precursor cells. VIM-D5 and VIM-2 inhibit the proliferation of clusters and colony formation after seven days of incubation. Day-14 CFU-GM are not affected by these antibodies. After complement-mediated cytolysis with VIM-2, the number of BFU-e was significantly reduced; however, this effect was largely abrogated by addition of leukocyte-conditioned medium to the cultures as an exogenous source of burst-promoting activity. Furthermore, the maturation of myeloid progenitor cells has been examined by delayed treatment with VIM-D5 and complement during the in vitro culture period. In these experiments a different maturation behavior of day-7 and day-14 CFU-GM was demonstrated. To study whether a cryptic carbohydrate structure is present on more immature CFU-GM, the effect of neuraminidase treatment of myeloid progenitor cells on reactivity with VIM-D5 was tested.
在本研究中，我们评估了针对髓系分化抗原的单克隆细胞毒性抗体与造血前体细胞的反应性。VIM-D5和VIM-2在孵育7天后可抑制集落的增殖和集落形成。第14天的CFU-GM不受这些抗体的影响。在用VIM-2进行补体介导的细胞溶解后，BFU-e的数量显著减少；然而，通过向培养物中添加白细胞条件培养基作为外源性爆式促进活性来源，这种效应在很大程度上被消除。此外，在体外培养期间，通过用VIM-D5和补体进行延迟处理来检查髓系祖细胞的成熟情况。在这些实验中，证明了第7天和第14天的CFU-GM具有不同的成熟行为。为了研究在更不成熟的CFU-GM上是否存在隐蔽的碳水化合物结构，测试了神经氨酸酶处理髓系祖细胞对与VIM-D5反应性的影响。
