真空辅助闭合治疗对耐甲氧西林金黄色葡萄球菌创面生物膜的影响。
The Effect of Vacuum-Assisted Closure Therapy on Methicillin-Resistant Staphylococcus aureus Wound Biofilms.
机构信息
In Belgrade, Serbia, Ivana Ćirković, MD, PhD, is Professor of Microbiology, Institute of Microbiology, University of Belgrade; Dario Jocić, MD, MS, is a vascular surgeon, Institute of Cardiovascular Diseases; Dragana D. Božić, MD, PhD, is Professor of Microbiology, Department of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade; Slobodanka Djukić, MD, PhD, is Professor of Microbiology, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade; Neda Konstantinović, MD, is a medical doctor, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade; and Djordje Radak, MD, PhD, is Chief of Vascular Surgery, Institute of Cardiovascular Diseases, and Professor of Surgery, Faculty of Medicine, University of Belgrade. Acknowledgments: This research was supported by the Ministry of Education, Science and Technological Development, Republic of Serbia (project no. ON175039).
出版信息
Adv Skin Wound Care. 2018 Aug;31(8):361-364. doi: 10.1097/01.ASW.0000540070.07040.70.
Biofilm-associated wound infections are a major global health issue, and methicillin-resistant Staphylococcus aureus (MRSA) is among the greatest therapeutic challenges. Vacuum-assisted closure (VAC) therapy is now being revisited as an alternative treatment for both acute and chronic wounds. However, data supporting the concept of its antibiofilm effect remain limited. Using quantitative biofilm-forming assay and a range of genotypic methods (spa, SCCmec, and agr typing), study authors showed that VAC therapy can significantly prevent biofilm formation (P < .01) of a range of MRSA wound isolates differing widely in their biofilm-forming abilities and genetic background. The best effect was presented on CC5-MRSA-SCCmecI-agrII, a dominant MRSA clone among wound isolates worldwide. An assessment of effects of different protocols on dressing changes (1 or 2 times per week) demonstrated significantly greater antibiofilm activity (P < .05) of 3-day dressing changes. These findings support the use of VAC therapy as a topical antibiofilm treatment for the effective management of wound healing.
生物膜相关的伤口感染是一个全球性的主要健康问题，耐甲氧西林金黄色葡萄球菌 (MRSA) 是治疗的最大挑战之一。负压封闭引流 (VAC) 治疗目前正在重新被视为治疗急性和慢性伤口的一种替代方法。然而，支持其抗生物膜作用的概念的数据仍然有限。研究作者使用定量生物膜形成测定法和一系列基因方法 (spa、SCCmec 和 agr 分型) 表明，VAC 治疗可显著预防一系列 MRSA 伤口分离株的生物膜形成 (P<.01)，这些分离株在生物膜形成能力和遗传背景方面差异很大。对 CC5-MRSA-SCCmecI-agrII（一种在世界各地伤口分离株中占主导地位的 MRSA 克隆）的效果评估表明，每周更换 1 或 2 次敷料的最佳效果。不同方案对敷料更换（每周 1 次或 2 次）的评估表明，3 天更换敷料具有显著更高的抗生物膜活性 (P<.05)。这些发现支持将 VAC 治疗作为一种局部抗生物膜治疗方法，用于有效管理伤口愈合。
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