Clinic for Otorhinolaryngology and Maxillofacial Surgery, Clinical Centre of Serbia, 11000, Belgrade, Serbia.
Faculty of Medicine, University of Belgrade, 11000, Belgrade, Serbia.
Eur Arch Otorhinolaryngol. 2016 Mar;273(3):615-20. doi: 10.1007/s00405-015-3607-8. Epub 2015 Mar 22.
Bacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. The aim of the study was to investigate whether biofilm formation on tympanostomy tubes depends on the genetic profile of methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on fluoroplastic tympanostomy tubes (TTs) was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on TTs. Tested MRSA strains were classified into SCCmec type I (36.7 %), III (23.3 %), IV (26.7 %) and V (13.3 %), agr type I (50 %), II (36.7 %) and III (13.3 %), and 5 clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on TTs was as following: 13.3 % of strains belonged to the category of no biofilm producers, 50 % to the category of weak biofilm producers and 36.7 % to moderate biofilm producers. There was a statistically significant difference between CC, SCCmec and agr types and the category of biofilm production on TTs tubes (p < 0.001): CC5, SCCmecI type and agrII type with a moderate amount of biofilm, and CC8 and agrI type with a low amount of biofilm. Biofilm formation by MRSA on TTs is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related post-tympanostomy tube otorrhea.
细菌生物膜的形成与鼓室置管后持续性耳漏的高发生率有关。本研究旨在探讨耐甲氧西林金黄色葡萄球菌（MRSA）菌株生物膜的形成是否取决于其基因谱。我们在 30 株 MRSA 菌株上测试了氟塑料鼓膜置管（TTs）上生物膜的形成能力。通过 nuc 和 mecA 基因的 PCR 确认鉴定和耐甲氧西林。对菌株进行基因分型（SCCmec、agr 和 spa 分型）。在微量滴定板和 TTs 上测试生物膜形成。测试的 MRSA 菌株分为 SCCmec 型 I（36.7%）、III（23.3%）、IV（26.7%）和 V（13.3%）、agr 型 I（50%）、II（36.7%）和 III（13.3%）和 5 个克隆复合体（CC）。所有测试的 MRSA 菌株均显示出在微量滴定板上形成生物膜的能力。TTs 上生物膜形成的能力如下：13.3%的菌株属于无生物膜产生菌类别，50%的菌株属于弱生物膜产生菌类别，36.7%的菌株属于中等生物膜产生菌类别。CC、SCCmec 和 agr 型与 TTs 管生物膜产生类别之间存在统计学差异（p<0.001）：CC5、SCCmecI 型和 agrII 型为中等量生物膜，CC8 和 agrI 型为少量生物膜。MRSA 在 TTs 上形成生物膜高度依赖于菌株的遗传特征。因此，MRSA 基因分型可能有助于确定与生物膜相关的鼓室置管后耳漏的可能性。
