Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Rio Ebro-Edificio I+D, C/Mariano Esquillor S/N, 50018 Zaragoza, Spain; Aragon Health Research Institute (IIS Aragon), 50009 Zaragoza, Spain.
Institute for Membrane Technology, National Research Council of Italy, ITM-CNR c/o University of Calabria, Via P. Bucci cubo 17/C, I-87036 Rende, Italy.
J Colloid Interface Sci. 2018 Dec 1;531:126-137. doi: 10.1016/j.jcis.2018.07.029. Epub 2018 Jul 18.
The development of novel scaffolds based on biocompatible polymers is of great interest in the field of bone repair for fabrication of biodegradable scaffolds that mimic the extracellular matrix and have osteoconductive and osteoinductive properties for enhanced bone regeneration.
Polycaprolactone (PCL) and polycaprolactone/polyvinyl acetate (PCL/PVAc) core-shell fibers were synthesised and decorated with poly(lactic-co-glycolic acid) [PLGA] particles loaded with bone morphogenetic protein 2 (BMP2) by simultaneous electrospinning and electrospraying. Hydroxyapatite nanorods (HAn) were loaded into the core of fibers. The obtained scaffolds were characterised by scanning and transmission electron microscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The in vitro potential of these materials for bone regeneration was assessed in biodegradation assays, osteoblast viability assays, and analyses of expression of specific bone markers, such as alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN).
PLGA particles were homogeneously distributed in the entire fibre mat. The growth factor load was 1.2-1.7 μg/g of the scaffold whereas the HAn load was in the 8.8-12.6 wt% range. These scaffolds were able to support and enhance cell growth and proliferation facilitating the expression of osteogenic and osteoconductive markers (OCN and OPN). These observations underline the great importance of the presence of BMP2 in scaffolds for bone remodelling as well as the good potential of the newly developed scaffolds for clinical use in tissue engineering.
基于生物相容性聚合物的新型支架的开发在骨修复领域具有重要意义，用于制造可生物降解的支架，这些支架模拟细胞外基质，具有骨传导和骨诱导特性，可增强骨再生。
合成了聚己内酯（PCL）和聚己内酯/聚醋酸乙烯酯（PCL/PVAc）核壳纤维，并通过同时静电纺丝和静电喷涂将其用载有骨形态发生蛋白 2（BMP2）的聚乳酸-共-羟基乙酸[PLGA]颗粒进行修饰。将羟基磷灰石纳米棒（HAn）加载到纤维的核心中。通过扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱和热重分析对获得的支架进行了表征。通过在生物降解试验、成骨细胞活力试验以及碱性磷酸酶（ALP）、骨钙素（OCN）和骨桥蛋白（OPN）等特定骨标志物表达分析中评估了这些材料在骨再生方面的体外潜力。
PLGA 颗粒均匀分布在整个纤维垫中。生长因子的负载量为 1.2-1.7μg/g 支架，而 HAn 的负载量在 8.8-12.6wt%范围内。这些支架能够支持和增强细胞生长和增殖，促进成骨和骨诱导标志物（OCN 和 OPN）的表达。这些观察结果强调了 BMP2 在支架中对骨重塑的重要性，以及新开发的支架在组织工程临床应用中的良好潜力。
