基于纳米金属表面能量转移平台的人尿液中精胺的高选择性检测。

Highly selective detection of spermine in human urine via a nanometal surface energy transfer platform.

机构信息

Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.

Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China; College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.

出版信息

Talanta. 2018 Oct 1;188:218-224. doi: 10.1016/j.talanta.2018.05.086. Epub 2018 May 25.

DOI:10.1016/j.talanta.2018.05.086

PMID:30029367

Abstract

As an important biomarker of malignant tumors, spermine is closely related with some diseases. In this work, a nanometal surface energy transfer (NSET) strategy via the positively charged gold nanorods (AuNRs) and the negatively charged tetrakis (4-sulfonatophenyl) porphyrin (TPPS) was developed to detect spermine in human urine. Under acidic condition, spermine possessed multi-cationic property and a strong affinity towards the anionic phosphate backbone of calf thymus DNA (ctDNA) by electrostatic attraction as well as the groove binding, which enabled to regulate the process of NSET between AuNRs and TPPS, leading to the fluorescence quenching of TPPS. Moreover, the quenched fluorescence was proportional to the concentration of spermine, which was applicable to monitor the level of spermine in human urine in the concentration range of 0.5-7.5 μM. The NSET platform for spemine is simple, selective and time-saving, which has great significance in early cancer diagnosis.

摘要

精胺作为恶性肿瘤的重要生物标志物，与某些疾病密切相关。在这项工作中，我们通过带正电荷的金纳米棒（AuNRs）和带负电荷的四（4-磺基苯基）卟啉（TPPS）开发了一种纳米金属表面能量转移（NSET）策略，用于检测人尿中的精胺。在酸性条件下，精胺具有多阳离子特性，并通过静电吸引以及沟槽结合与小牛胸腺 DNA（ctDNA）的阴离子磷酸骨架具有很强的亲和力，从而能够调节 AuNRs 和 TPPS 之间的 NSET 过程，导致 TPPS 的荧光猝灭。此外，猝灭的荧光与精胺的浓度成正比，可用于在 0.5-7.5 μM 的浓度范围内监测人尿中精胺的水平。用于精胺的 NSET 平台简单、选择性强且节省时间，在早期癌症诊断中具有重要意义。