枯草芽孢杆菌重组噬菌体基因组中tmrA7突变导致amyE⁺-tmrB⁺基因区域的扩增和缺失

Amplification and deletion of the amyE+-tmrB+ gene region in a Bacillus subtilis recombinant-phage genome by the tmrA7 mutation.

作者信息

Furusato T, Takano J, Yamane K, Hashiguchi K, Tanimoto A, Mori M, Yoda K, Yamasaki M, Tamura G

出版信息

J Bacteriol. 1986 Feb;165(2):549-56. doi: 10.1128/jb.165.2.549-556.1986.

DOI:10.1128/jb.165.2.549-556.1986

PMID:3003030

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214454/

Abstract

A 22.4-kilobase DNA fragment containing the tmrA7-amyR2-amyE+-tmrB+-aroI+ region of the Bacillus subtilis N7 chromosomal DNA was cloned into a recombinant B. subtilis bacteriophage, p11-AA248. The amyE+-tmrB+ gene region, approximately 12.6 kilobases, in the phage genome was amplified in a tunicamycin-resistant (Tmr) Amy+ AroI+ transductant of B. subtilis by p11-AA248. On the other hand, the amyE+-tmrB+ region in the genomes of 80 to 90% of the phage particles was deleted when the phages were induced from the Tmr Amy+ AroI+ transductants by treatment with 1.0 micrograms of mitomycin C per ml. From analyses of the physical maps and DNA nucleotide sequences in the junction region of the deleted phage genome and the parental DNA fragments, it is suggested that the deletion occurred within a direct repeat sequence composed of 18 base pairs. The endpoints of the amplified gene region seemed to be closely related to both terminal regions of the deleted DNA.

摘要

将含有枯草芽孢杆菌N7染色体DNA的tmrA7 - amyR2 - amyE⁺ - tmrB⁺ - aroI⁺区域的一个22.4千碱基的DNA片段克隆到重组枯草芽孢杆菌噬菌体p11 - AA248中。噬菌体基因组中约12.6千碱基的amyE⁺ - tmrB⁺基因区域在枯草芽孢杆菌的抗衣霉素（Tmr）Amy⁺ AroI⁺转导子中被p11 - AA248扩增。另一方面，当用每毫升1.0微克丝裂霉素C处理从Tmr Amy⁺ AroI⁺转导子诱导出的噬菌体时，80%至90%的噬菌体颗粒基因组中的amyE⁺ - tmrB⁺区域被删除。通过对缺失噬菌体基因组与亲本DNA片段连接区域的物理图谱和DNA核苷酸序列分析，表明缺失发生在一个由18个碱基对组成的直接重复序列内。扩增基因区域的端点似乎与缺失DNA的两个末端区域密切相关。