Tlsty T D, Albertini A M, Miller J H
Cell. 1984 May;37(1):217-24. doi: 10.1016/0092-8674(84)90317-9.
We have characterized strains of E. coli in which the lac region, together with varying amounts of surrounding DNA, is amplified 40 to 200 fold. The amplification events involve regions of 7 to 37 kb and result in a tandem array of repeated units. Restriction digest patterns of DNA from over 100 independent strains reveal that the amplified units are different in each case. Mechanisms of gene duplication and amplification, and the relationship of gene amplification in bacteria to that in eucaryotic cells, are considered.
我们已经对大肠杆菌菌株进行了特性分析，其中乳糖操纵子区域以及不同数量的周边DNA被扩增了40至200倍。扩增事件涉及7至37kb的区域，并导致重复单元的串联排列。来自100多个独立菌株的DNA限制性消化模式表明，每种情况下扩增的单元都是不同的。我们还考虑了基因复制和扩增的机制，以及细菌中的基因扩增与真核细胞中的基因扩增之间的关系。
