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C3-reacted sepharose: a preparative method for separating T and B lymphocytes.C3反应琼脂糖：一种分离T淋巴细胞和B淋巴细胞的制备方法。
Clin Exp Immunol. 1977 Jan;27(1):38-42.
2
Separation of T lymphocytes from normal individuals and patients with B lymphocyte chronic lymphocytic leukaemia.从正常个体和B淋巴细胞慢性淋巴细胞白血病患者中分离T淋巴细胞。
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Fractionation of human blood lymphocytes on Helix pomatia A haemagglutinin coupled to sepharose beads.利用与琼脂糖珠偶联的苹果扁卷螺A血凝素对人血淋巴细胞进行分级分离。
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Human T and B lymphocytes separation on Sepharose C-3 " activated " columns.在琼脂糖C-3“活化”柱上分离人T淋巴细胞和B淋巴细胞。
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Xenoantisera against a murine T cell tumor product cross-react with immunoglobulin Fab determinants.针对小鼠T细胞肿瘤产物的异种抗血清与免疫球蛋白Fab决定簇发生交叉反应。
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6
Functional analysis of B cell subpopulations: responsiveness of phenotypically characterized CR+ and CR- B cell subsets to a T-dependent antigen in vitro.B细胞亚群的功能分析：表型特征明确的补体受体阳性（CR+）和补体受体阴性（CR-）B细胞亚群在体外对T细胞依赖性抗原的反应性。
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7
IgM complex receptors on subpopulations of murine lymphocytes.小鼠淋巴细胞亚群上的IgM复合受体。
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Enumeration and isolation of rabbit T and B lymphocytes by using antibody-coated erythrocytes.利用抗体包被的红细胞对兔T淋巴细胞和B淋巴细胞进行计数与分离。
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In vitro activation of human T and B lymphocytes by pokeweed mitogen.美洲商陆丝裂原对人T淋巴细胞和B淋巴细胞的体外激活作用。
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引用本文的文献

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In vitro generation of human cytotoxic lymphocytes by virus. Viral glycoproteins induce nonspecific cell-mediated cytotoxicity without release of interferon.病毒在体外诱导人细胞毒性淋巴细胞的产生。病毒糖蛋白诱导非特异性细胞介导的细胞毒性，且不释放干扰素。
J Exp Med. 1981 Sep 1;154(3):840-55. doi: 10.1084/jem.154.3.840.

本文引用的文献

1
Inhibition by C3 fragments of C3-dependent rosette formation and antigen-induced lymphocyte transformation.C3 片段对 C3 依赖性玫瑰花结形成及抗原诱导的淋巴细胞转化的抑制作用。
Clin Exp Immunol. 1974 Oct;18(2):273-82.
2
Cellular immunoadsorbents: a simplified technique for separation of lymphoid cell populations.细胞免疫吸附剂：一种分离淋巴细胞群体的简化技术。
J Immunol. 1974 Mar;112(3):1231-43.
3
The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulins.淋巴细胞膜的动态状态。影响表面免疫球蛋白分布和更新的因素。
Eur J Immunol. 1972 Jun;2(3):203-12. doi: 10.1002/eji.1830020304.
4
Specific purification of lymphocyte populations on a digestible immunoabsorbent.在可消化免疫吸附剂上对淋巴细胞群体进行特异性纯化。
J Immunol. 1973 Jan;110(1):313-9.
5
Human peripheral lymphocytes bearing both B-cell complement receptors and T-cell characteristics for sheep erythrocytes detected by a mixed rosette method.用混合玫瑰花结法检测到的同时带有B细胞补体受体和对绵羊红细胞具有T细胞特性的人外周淋巴细胞。
Clin Exp Immunol. 1974 Dec;18(4):483-90.
6
Combined detection of human T and B lymphocytes by rosette formation with sheep erythrocytes and zymosan-C3 complexes.通过与绵羊红细胞和酵母聚糖-C3复合物形成花环联合检测人T淋巴细胞和B淋巴细胞
J Immunol. 1974 Aug;113(2):531-6.
7
Binding of soluble immune complexes to human lymphoblastoid cells. I. Characterization of receptors for IgG Fc and complement and description of the binding mechanism.可溶性免疫复合物与人淋巴母细胞的结合。I. IgG Fc和补体受体的特性及结合机制的描述。
J Exp Med. 1974 Oct 1;140(4):877-94. doi: 10.1084/jem.140.4.877.
8
Fate of HL-A antigens and antibodies at the lymphocyte surface.淋巴细胞表面HL-A抗原和抗体的命运
Transplant Proc. 1973 Dec;5(4):1835-47.
9
Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.人类T和B淋巴细胞的表面标志物。I. 大量与绵羊红细胞形成非免疫性玫瑰花结的淋巴细胞。
J Exp Med. 1972 Aug 1;136(2):207-15. doi: 10.1084/jem.136.2.207.
10
Separation and functional analysis of subpopulations of lymphocytes bearing complement and Fc receptors.携带补体和Fc受体的淋巴细胞亚群的分离与功能分析。
Transplant Rev. 1975;25:98-120. doi: 10.1111/j.1600-065x.1975.tb00727.x.

C3反应琼脂糖：一种分离T淋巴细胞和B淋巴细胞的制备方法。

C3-reacted sepharose: a preparative method for separating T and B lymphocytes.

作者信息

Casali P, Perussia B M

出版信息

Clin Exp Immunol. 1977 Jan;27(1):38-42.

PMID:300306
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1540909/
Abstract

Lymphocytes bearing complement receptors were separated from lymphocyte suspensions using C3-reacted Sepharose columns as solid phase immunoabsorbents. The retained CR+ lymphocytes were recovered by elution of the column with IgG fraction purified from a rabbit antiserum to human C3. This paper describes the method, its specificity and the yields of cells obtained.

摘要

使用与C3反应的琼脂糖柱作为固相免疫吸附剂，从淋巴细胞悬液中分离出带有补体受体的淋巴细胞。通过用从兔抗人C3血清中纯化的IgG组分洗脱柱子，回收保留的CR+淋巴细胞。本文描述了该方法、其特异性以及获得的细胞产量。