Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147 002, Punjab, India.
Pharmaceutics Division, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, Punjab, India.
Int J Biol Macromol. 2018 Oct 15;118(Pt B):2245-2255. doi: 10.1016/j.ijbiomac.2018.07.107. Epub 2018 Jul 18.
Corneal injuries are the major problem arises these days due to excessive use of mobile phone, TV, environment pollution, etc. Thus, a need is to evaluate materials having good corneal wound healing potential. To achieve this goal, pullulan was derivatized using carbamoylethylation and 2 factorial design has been found a good approach for the optimization of synthesis process of carbamoylethyl pullulan (CMEP). The design expert software applied for this purpose suggested significance of the generated model. ATR-FTIR, NMR and XRD spectral attributes validated the structure of CMEP. Interestingly, derivatization of pullulan to CMEP has increased its ΔH from 171.552 J/g to 203.5 J/g and decreased the endothermic transition from 323.44 °C to 248.67 °C. Further, rheological behavior suggested enhanced viscosity after carbamoylethylation indicating increase in H-bonding that further participate in the enhancement of corneal adhesive strength. The CMEP possessed high corneal wound healing property that was associated with its high corneal adhesive strength and oxygen neutralizing capacity. Thus, the findings suggested overwhelming influence of CMEP as a corneal wound healing agent.
由于过度使用手机、电视、环境污染等因素，角膜损伤是当今主要的问题。因此，需要评估具有良好角膜伤口愈合潜力的材料。为了实现这一目标，使用氨乙基化对普鲁兰进行了衍生化，并且发现 2 因子设计是优化氨乙基普鲁兰（CMEP）合成工艺的有效方法。为此目的应用了设计专家软件来建议生成模型的重要性。ATR-FTIR、NMR 和 XRD 光谱属性验证了 CMEP 的结构。有趣的是，普鲁兰衍生化为 CMEP 后，其 ΔH 从 171.552 J/g 增加到 203.5 J/g，而吸热转变从 323.44°C 降低到 248.67°C。此外，流变行为表明氨乙基化后粘度增加，表明氢键增加，进一步参与增强角膜黏附强度。CMEP 具有较高的角膜伤口愈合性能，这与其较高的角膜黏附强度和氧中和能力有关。因此，研究结果表明 CMEP 作为角膜伤口愈合剂具有重要的影响。
