Singh R S, Kaur Navpreet
Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, Punjab 147 002 India.
Food Sci Biotechnol. 2019 Mar 5;28(5):1507-1520. doi: 10.1007/s10068-019-00585-w. eCollection 2019 Oct.
Central composite rotatable design of RSM was used for the optimization of medium composition for pullulan production from de-oiled rice bran by in shake-flask fermentations. The sugars from de-oiled rice bran were extracted in distilled water under moist steam pressure and the obtained de-oiled rice bran extract (DRBE) was used for the optimization of medium composition. RSM optimized medium components (DRBE sugars, 3.88%; yeast extract, 0.24%; (NH)SO, 0.06%; KHPO, 0.57% (w/v), and pH, 5.22) supported 5.48% (w/v) pullulan production and 0.88 (A/100) biomass yield. Coefficient of determination for pullulan production (0.99) and biomass yield (0.99) was close to 1.0 which justifies significance of model. Lack of fit for both responses was non-significant, which shows fitness of quadratic model. FTIR and NMR spectral attributes confirmed the structure of pullulan. XRD patterns verified the amorphous nature of pullulan. De-oiled rice bran was found as a potential substrate for pullulan production.
响应曲面法的中心复合旋转设计用于优化在摇瓶发酵中从脱脂米糠生产普鲁兰多糖的培养基组成。在湿蒸汽压力下，将脱脂米糠中的糖分在蒸馏水中提取，所得的脱脂米糠提取物（DRBE）用于培养基组成的优化。响应曲面法优化的培养基成分（DRBE糖，3.88%；酵母提取物，0.24%；(NH₄)₂SO₄，0.06%；KH₂PO₄，0.57%（w/v），pH值为5.22）支持5.48%（w/v）的普鲁兰多糖产量和0.88（A/100）的生物量产量。普鲁兰多糖产量（0.99）和生物量产量（0.99）的决定系数接近1.0，这证明了模型的显著性。两个响应的失拟均不显著，这表明二次模型的适用性。傅里叶变换红外光谱（FTIR）和核磁共振（NMR）光谱属性证实了普鲁兰多糖的结构。X射线衍射（XRD）图谱验证了普鲁兰多糖的无定形性质。发现脱脂米糠是生产普鲁兰多糖的潜在底物。
