Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkuric, hi-627 412 Tirunelveli, Tamil Nadu, India.
Molecular Entomology Laboratory, Department of Biotechnology, School of Biosciences, Periyar University, Salem 636 011, Tamil Nadu, India.
Pestic Biochem Physiol. 2018 Jul;149:54-60. doi: 10.1016/j.pestbp.2018.05.009. Epub 2018 May 23.
Insects have developed tolerance against mycoses caused by entomopathogenic fungi through several humoral and cellular mechanisms. Antioxidant enzymes such as superoxide dismutase, lipid peroxidase, and peroxidase can play a role in defense against mycosis, but the physiological interactions between the fungus and the insect are not well characterized. In this study, the effects of infection by entomopathogenic fungus, Aspergillus flavus on the antioxidant defense system of Spodoptera litura, were investigated. The fungi, A. flavus exposure resulted in modification of the levels of antioxidant enzymes, as well as significant decline in phenoloxidase titers and the total hemocyte count 48 h post exposure. A significant increase was observed in detoxifying enzymes. All these results suggest that A. flavus infects S. litura by directly acting on the immune system, resulting in decreased immune function. Bioassay results showed that A. flavus affects third and fourth instar larvae of S. litura. This report supports the importance of A. flavus as a candidate for biological control of S. litura.
昆虫通过几种体液和细胞机制对由昆虫病原真菌引起的真菌感染产生了耐受性。超氧化物歧化酶、脂质过氧化物酶和过氧化物酶等抗氧化酶可以在抗真菌病中发挥作用，但真菌与昆虫之间的生理相互作用尚未得到很好的描述。在这项研究中，研究了昆虫病原真菌黄曲霉对斜纹夜蛾抗氧化防御系统的影响。结果表明，黄曲霉真菌暴露会改变抗氧化酶的水平，并在暴露后 48 小时内显著降低酚氧化酶效价和总血细胞计数。解毒酶的含量显著增加。所有这些结果表明，黄曲霉通过直接作用于免疫系统感染斜纹夜蛾，导致免疫功能下降。生物测定结果表明，黄曲霉影响斜纹夜蛾的三龄和四龄幼虫。本报告支持黄曲霉作为斜纹夜蛾生物防治候选物的重要性。
