Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical & Technical Sciences, Saveetha University, Chennai, Tamil Nadu, 600077, India.
Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.
Sci Rep. 2022 Oct 6;12(1):16775. doi: 10.1038/s41598-022-20426-x.
Insect pests of agricultural crops have establish immunological tolerance against fungal infection caused by pathogens via different humoral and cellular processes. Fungal infection can be prevented by insect antioxidant and detoxifying enzymes, but there is no clear understanding of how they physiologically and biochemically interact. Our study aims to examine the antioxidant and detoxifying enzyme defense systems of the pest insect Spodoptera litura in response to infection by Metarhizium flavoviride. At 48 h following exposure to M. flavoviride, antioxidant enzyme levels were modified, and phenoloxidase and total hemocyte count were decreased significantly. The amount of detoxifying enzymes increased significantly. M. flavoviride appears to directly affect the S. litura immune system and results in decreased immunity. In a bioassay, M. flavoviride was found to be harmful to S. litura larvae in their third and fourth instar stage. M. flavoviride may be an effective tool in the control of S. litura larvae. Such entomopathogenic fungi represent cheaper, pollution free, target specific, promising alternatives to synthetic chemical tools in the for control insect pests.
农业作物害虫通过不同的体液和细胞过程对病原体引起的真菌感染建立了免疫耐受。昆虫抗氧化和解毒酶可以预防真菌感染，但对于它们在生理和生化上如何相互作用还没有明确的认识。我们的研究旨在研究鳞翅目夜蛾（Spodoptera litura）在感染玫烟色棒束孢（Metarhizium flavoviride）后对其抗氧化和解毒酶防御系统的影响。在暴露于玫烟色棒束孢 48 小时后，抗氧化酶水平发生改变，酚氧化酶和总血细胞计数显著降低。解毒酶的含量显著增加。玫烟色棒束孢似乎直接影响鳞翅目夜蛾的免疫系统，导致免疫力下降。在生物测定中，发现玫烟色棒束孢对三、四龄鳞翅目夜蛾幼虫有危害作用。玫烟色棒束孢可能是防治鳞翅目夜蛾幼虫的有效工具。这类昆虫病原真菌作为防治害虫的替代物，代表着更廉价、无污染、靶向明确、有前景的合成化学工具。
