Mackman N, Nicaud J M, Gray L, Holland I B
Mol Gen Genet. 1985;201(2):282-8. doi: 10.1007/BF00425672.
We have identified gene products corresponding to hlyC, hlyA and hlyD encoded by the Escherichia coli haemolytic determinant 2001 of human origin cloned into the recombinant plasmid pLG570. The product of hlyC is required for the "activation" of the inactive 107K polypeptide encoded by the hlyA gene. The activated 107K protein constitutes the active haemolysin secreted into the medium. hlyB and hlyD are separate regions defined by complementation studies and encode functions essential for the export of haemolysin with hlyD encoding a 53K protein. Complementation studies using subclones and Tn5 insertions into pLG570 have revealed the presence of two major promoters upstream of hlyC and hlyD which transcribe the four hly genes in the same direction. Finally, we were able to reconstitute the complete haemolysin system from three different plasmids encoding hlyC, hlyA and hlyB + hlyD, respectively.
我们已鉴定出与克隆到重组质粒pLG570中的人源大肠杆菌溶血决定簇2001所编码的hlyC、hlyA和hlyD相对应的基因产物。hlyC的产物是hlyA基因所编码的无活性107K多肽“激活”所必需的。被激活的107K蛋白构成分泌到培养基中的活性溶血素。hlyB和hlyD是通过互补研究确定的独立区域，它们编码溶血素输出所必需的功能，其中hlyD编码一种53K蛋白。使用亚克隆和向pLG570中插入Tn5进行的互补研究表明，在hlyC和hlyD上游存在两个主要启动子，它们以相同方向转录四个hly基因。最后，我们能够从分别编码hlyC、hlyA和hlyB + hlyD的三种不同质粒中重建完整的溶血素系统。
