a Department of Chemistry (Organic chemistry), Miyaneh Branch , Islamic Azad University , Miyaneh , Iran.
b Department of Chemistry , Payame Noor University , Tehran , Iran.
J Biomater Sci Polym Ed. 2019 Jan;30(1):12-33. doi: 10.1080/09205063.2018.1504193. Epub 2019 Jan 11.
Currently an effective strategy in nanomedicine for cancer therapy is the combination of photothermal therapy with chemotherapy. Because combination cancer therapy improve the therapy efficiency by synergistic effects and overcoming drug resistance as compared to monotherapy possesses. According to these facts, gold nanorods-cored biodegradable micelles were prepared by coating gold nanorods (AuNRs) with synthesized pH-sensitive thiol-ended amphiphilic triblock copolymer (PAA-b-PDMAEMAQ-b-PCL-SH). The synthesized AuNRs@polymer was loaded with methotrexate (MTX) as an anticancer drug through electrostatic interactions to afford AuNRs@polymer-MTX. The success of the coating was investigated by means of atomic force microscopy (AFM), thermogravimetric analysis (TGA), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, ultraviolet-visible (UV-vis) spectroscopy, as well as dynamic light scattering (DLS), and zeta potential measurements. MTX-loading capacity, and pH triggered in vitro drug release behavior of the synthesized nanocomposites were also investigated. In vitro cytotoxic effects was comprehensively evaluated among free MTX, AuNRs@polymer, and AuNRs@polymer-MTX, with or without NIR light irradiation (1064 nm, 125 mJ/pulse, and 4 min) to improve curative effect of AuNRs@polymer-MTX led by the combination of photothermal therapy and chemotherapy.
目前，癌症治疗中一种有效的纳米医学策略是将光热疗法与化学疗法相结合。因为与单一疗法相比，联合癌症疗法通过协同作用和克服耐药性来提高治疗效率。基于这些事实，通过用合成的 pH 敏感的硫醇封端两亲性三嵌段共聚物（PAA-b-PDMAEMAQ-b-PCL-SH）包覆金纳米棒（AuNRs），制备了金纳米棒核可生物降解的胶束。通过静电相互作用将合成的 AuNRs@聚合物负载甲氨蝶呤（MTX）作为抗癌药物，以提供 AuNRs@聚合物-MTX。通过原子力显微镜（AFM）、热重分析（TGA）、透射电子显微镜（TEM）、傅里叶变换红外（FTIR）光谱、紫外可见（UV-vis）光谱以及动态光散射（DLS）和zeta 电位测量来研究涂层的成功。还研究了合成的纳米复合材料的载药量和 pH 触发的体外药物释放行为。在没有或有近红外光照射（1064nm，125mJ/脉冲，4 分钟）的情况下，综合评估了游离 MTX、AuNRs@聚合物和 AuNRs@聚合物-MTX 之间的体外细胞毒性作用，以提高光热疗法和化学疗法联合治疗 AuNRs@聚合物-MTX 的疗效。
