Kerner J, Bieber L L
Prep Biochem. 1985;15(4):237-57. doi: 10.1080/00327488508062443.
Medium-chain acylcarnitines were isolated from human urine using a combination of chloroform-methanol extraction, silicic acid column and molecular sieving chromatography and preparative HPLC. Three purified acylcarnitines were analyzed by fast atom bombardment mass spectrometry and were also saponified and the free fatty acids analyzed by gas chromatography and mass spectrometry. Combined electron impact mass spectrometry and fast atom bombardment mass spectrometry and periodate oxidation for location of double bonds, demonstrated the occurrence of delta 6-octenylcarnitine, 2-methyloctanylcarnitine and 2-methyl-delta 6-octenylcarnitine. These acylcarnitines were present in the thirteen urines obtained from normal humans, but were not detected in urines from three individuals who had been on total parenteral nutrition for more than a year. The occurrence of alpha-methyl medium-chain acylcarnitines in human urine indicates a role for carnitine in excretion (detoxification) of these acyl derivatives.
采用氯仿 - 甲醇萃取、硅酸柱、分子筛色谱和制备型高效液相色谱相结合的方法，从人尿中分离出中链酰基肉碱。通过快原子轰击质谱对三种纯化的酰基肉碱进行分析，同时将其皂化，并通过气相色谱和质谱对游离脂肪酸进行分析。结合电子轰击质谱、快原子轰击质谱以及用于双键定位的高碘酸盐氧化法，证实了δ6 - 辛烯基肉碱、2 - 甲基辛酰基肉碱和2 - 甲基 - δ6 - 辛烯基肉碱的存在。这些酰基肉碱存在于从正常人获取的13份尿液中，但在接受全胃肠外营养超过一年的三名个体的尿液中未检测到。人尿中α - 甲基中链酰基肉碱的出现表明肉碱在这些酰基衍生物的排泄（解毒）中发挥作用。
