Mannino Robert G, Pradhan Pallab, Roy Krishnendu, Lam Wilbur A
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, United States; Department of Pediatrics, Division of Pediatric Hematology/Oncology Aflac Cancer Center and Blood Disorders Center of Children's Healthcare of Atlanta, Emory University School of Medicine, Atlanta, GA, United States.
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, United States; Department of Pediatrics, Division of Pediatric Hematology/Oncology Aflac Cancer Center and Blood Disorders Center of Children's Healthcare of Atlanta, Emory University School of Medicine, Atlanta, GA, United States.
Methods Cell Biol. 2018;146:149-158. doi: 10.1016/bs.mcb.2018.05.007. Epub 2018 Jun 25.
Diffuse large B-cell lymphoma (DLBCL) is a particularly aggressive cancer, impacting the lives of approximately 20,000 people annually in the United States. Elucidating cellular interactions that occur within the microenvironment of DLBCL tumors is crucial to the successful development of therapeutic strategies for this condition. As the in vivo microenvironment of DLBCL is quite complex and variable, in vitro platforms that can sufficiently recapitulate these multifaceted cellular interactions without introducing the complexities of in vivo systems are vital for understanding the pathophysiology of this disease. In this chapter, we present a method for fabrication and development of an in vitro DLBCL-on-chip model in which a fully vascularized, perfusable, microfluidic traverses a DLBCL tumor cell-laden hydrogel that successfully recapitulates hallmark attributes and cellular interaction that occur within the DLBCL tumor microenvironment. As this microfluidic approach makes use of common laboratory items and does not require traditional photolithography to fabricate, this system represents a vital tool that can unlock previously inaccessible research areas of the DLBCL tumor microenvironment to researchers across numerous fields.
弥漫性大B细胞淋巴瘤（DLBCL）是一种侵袭性特别强的癌症，在美国每年影响约20000人的生活。阐明DLBCL肿瘤微环境中发生的细胞相互作用对于成功开发针对这种疾病的治疗策略至关重要。由于DLBCL的体内微环境非常复杂且多变，能够充分重现这些多方面细胞相互作用而不引入体内系统复杂性的体外平台对于理解这种疾病的病理生理学至关重要。在本章中，我们介绍一种体外DLBCL芯片模型的制造和开发方法，其中一个完全血管化、可灌注的微流体穿过载有DLBCL肿瘤细胞的水凝胶，该水凝胶成功重现了DLBCL肿瘤微环境中发生的标志性特征和细胞相互作用。由于这种微流体方法使用常见的实验室物品，且不需要传统光刻技术来制造，该系统是一种重要工具，可为众多领域的研究人员打开DLBCL肿瘤微环境中以前无法进入的研究领域。
