Wallner S F, Vautrin R
Proc Soc Exp Biol Med. 1986 Jan;181(1):144-50. doi: 10.3181/00379727-181-42236.
The anemia of thermal injury is a multifactorial process and includes hemorrhage and hemolysis. Much evidence suggests that a reduced rate of erythropoiesis contributes to this anemia. Prior studies show that this anemia is temporally related to the appearance in burn patients sera of a substance(s) capable of inhibiting erythropoiesis in vitro. Four experiments were done to elucidate the mechanism of action of this inhibitor. In all experiments sera from burn patients previously shown to be inhibitory to erythropoiesis in vitro were studied. In the first, inhibitory sera were exposed to erythropoietin solutions without loss of erythropoietic activity. Second, mouse marrow cells were preincubated with serum without loss of their ability to form erythroid colonies. Third, the inhibitory effect could not be overcome with increasing amounts of erythropoietin. Finally, erythroid colony formation was effected only if the inhibitory serum was present during the first 8 to 12 hr of culture. The data suggest that the erythropoietic inhibitor in these sera acts directly on erythroid stem cells in vitro and not by inactivating or interference with erythropoietin.
热损伤性贫血是一个多因素过程，包括出血和溶血。大量证据表明，红细胞生成率降低是导致这种贫血的原因之一。先前的研究表明，这种贫血与烧伤患者血清中出现能够在体外抑制红细胞生成的物质在时间上相关。进行了四项实验以阐明这种抑制剂的作用机制。在所有实验中，均研究了先前已证明在体外对红细胞生成有抑制作用的烧伤患者血清。第一项实验中，将抑制性血清与促红细胞生成素溶液接触，促红细胞生成活性并未丧失。第二项实验中，小鼠骨髓细胞与血清预孵育，其形成红系集落的能力并未丧失。第三项实验中，增加促红细胞生成素的量并不能克服抑制作用。最后，只有在培养的最初8至12小时内存在抑制性血清时，红系集落形成才会受到影响。数据表明，这些血清中的红细胞生成抑制剂在体外直接作用于红系干细胞，而非通过使促红细胞生成素失活或干扰促红细胞生成素发挥作用。
