Shearer G M, Simpson E, Weinstein Y, Melmon K L
J Immunol. 1977 Mar;118(3):756-61.
Spleen cells from normal BALB/c mice were cultured in vitro with irradiated C57BL/6 stimulating cells. Five days later the T cell-mediated cytotoxic activity of the effector cells was assessed with a 51Cr-release assay that used H-2bEL-4 tumor cells as targets. Before the BALB/c responding lymphocytes were sensitized they were fractionated by passing the spleen cells over insolubilized histamine rabbit serum albumin Sepharose columns (H-RSA-S) or over rabbit serum albumin Sepharose (RSA-S) control columns. Fractionation of cells over the H-RSA-S columns depleted or significantly reduced the cytotoxic potential of the unretained cells. All cytotoxic potential was recovered when the cells that adhered to the H-RSA-S were eluted from the columns. In contrast, no effect on responsiveness was detected after the cells had been fractionated over the control column. The loss of response potential by the cells that did not adhere to H-RSA-S could not be accounted for by removal of macrophages nor by the concentration of cells with suppressor activity in the effluent. These cell fractionation studies raise the possiblity but do not prove that cytotoxic precursor cells may express amine receptors that could be responsible for their retention by insolubilized histamine columns.
将正常BALB/c小鼠的脾细胞与经辐照的C57BL/6刺激细胞在体外进行培养。五天后，用51Cr释放试验评估效应细胞的T细胞介导的细胞毒性活性，该试验以H-2b EL-4肿瘤细胞作为靶细胞。在BALB/c反应性淋巴细胞致敏之前，将脾细胞通过不溶性组胺兔血清白蛋白琼脂糖柱（H-RSA-S）或兔血清白蛋白琼脂糖（RSA-S）对照柱进行分离。通过H-RSA-S柱对细胞进行分离，使未保留的细胞的细胞毒性潜力降低或显著降低。当从柱上洗脱粘附在H-RSA-S上的细胞时，所有细胞毒性潜力都得以恢复。相比之下，细胞通过对照柱分离后，未检测到对反应性的影响。未粘附在H-RSA-S上的细胞反应潜力的丧失，既不能通过去除巨噬细胞来解释，也不能通过流出物中具有抑制活性的细胞的浓缩来解释。这些细胞分离研究提出了细胞毒性前体细胞可能表达胺受体的可能性，但并未证明这些受体可能是其被不溶性组胺柱保留的原因。
