从脑、脊髓和脑膜中分离白细胞用于流式细胞术分析。
Leukocyte Isolation from Brain, Spinal Cord, and Meninges for Flow Cytometric Analysis.
作者信息
Manglani Monica, Gossa Selamawit, McGavern Dorian B
机构信息
National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland.
Medical Scientist Training Program, Penn State College of Medicine, Hershey, Pennsylvania.
出版信息
Curr Protoc Immunol. 2018 Apr;121(1):e44. doi: 10.1002/cpim.44.
Abstract
A continual dialogue exists between the central nervous system (CNS) and immune system that contributes to neural homeostasis as well as protection from microbes, repair following damage, autoimmune disease, and neurodegeneration. Characterization of resident and peripherally derived leukocyte populations within the central nervous system can provide valuable information regarding how these cells contribute to steady-state and inflammatory conditions. Flow cytometry provides a method to conduct detailed multi-parameter analyses of immune cells isolated from various tissues. This protocol provides a method to isolate leukocytes from brain, spinal cord, and meninges for flow cytometric analysis and provides a basic framework for phenotyping these cells. © 2018 by John Wiley & Sons, Inc.
摘要
中枢神经系统（CNS）与免疫系统之间存在持续对话，这有助于神经稳态以及抵御微生物、损伤后修复、自身免疫性疾病和神经退行性变。对中枢神经系统内驻留和外周来源的白细胞群体进行表征，可以提供有关这些细胞如何促成稳态和炎症状态的有价值信息。流式细胞术提供了一种对从各种组织中分离出的免疫细胞进行详细多参数分析的方法。本方案提供了一种从脑、脊髓和脑膜中分离白细胞以进行流式细胞术分析的方法，并为这些细胞的表型分析提供了一个基本框架。© 2018约翰威立国际出版公司。
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