Ferreira David A, Cruz Rita, Venâncio Carlos, Faustino-Rocha Ana I, Silva Aura, Mesquita João R, Ortiz Ana L, Vala Helena
Department of Veterinary Medicine, ICAAM Research Center, University of Évora, 7006-554 Évora, Portugal.
Educational, Technologies and Health Study Center (CI&DETS), Polytechnic Institute of Viseu, 3504-510 Viseu, Portugal.
J Vet Sci. 2018 Sep 30;19(5):608-619. doi: 10.4142/jvs.2018.19.5.608.
This work aimed to evaluate the effects on renal tissue integrity after hydroxyethyl starch (HES) 130/0.4 and Ringer's lactate (RL) administration in pigs under general anesthesia after acute bleeding. A total of 30 mL/kg of blood were passively removed from the femoral artery in two groups of Large White pigs, under total intravenous anesthesia with propofol and remifentanil. After bleeding, Group 1 (n = 11) received RL solution (25 mL/kg) and Group 2 (n = 11) received HES 130/0.4 solution (20 mL/kg). Additionally, Group 3 (n = 6) was not submitted to bleeding or volume replacement. Pigs were euthanized and kidneys were processed for histopathological and immunohistochemical analyses. Minimal to moderate glomerular, tubular, and interstitial changes, as well as papillary necrosis, were observed in all experimental groups. Pre-apoptosis and apoptosis indicators were higher in pigs that received HES 130/0.4, indicating a higher renal insult. Both HES 130/0.4 and RL administration may cause renal injury, although renal injury may be more significant in pigs receiving HES 13/0.4. Results also suggest that total intravenous anesthesia with propofol and remifentanil may cause renal injury, and this effect can be dose related.
本研究旨在评估急性出血后在全身麻醉状态下给予猪羟乙基淀粉（HES）130/0.4和乳酸林格氏液（RL）后对肾组织完整性的影响。在两组大白猪中，通过股动脉被动放血30 mL/kg，麻醉采用丙泊酚和瑞芬太尼全静脉麻醉。放血后，第1组（n = 11）接受RL溶液（25 mL/kg），第2组（n = 11）接受HES 130/0.4溶液（20 mL/kg）。此外，第3组（n = 6）未进行放血或容量替代。猪处死后，取肾脏进行组织病理学和免疫组织化学分析。在所有实验组中均观察到轻度至中度的肾小球、肾小管和间质变化以及乳头坏死。接受HES 130/0.4的猪中凋亡前期和凋亡指标更高，表明肾损伤更严重。给予HES 130/0.4和RL均可能导致肾损伤，尽管接受HES 13/0.4的猪肾损伤可能更显著。结果还表明，丙泊酚和瑞芬太尼全静脉麻醉可能导致肾损伤，且这种效应可能与剂量有关。
