1 Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Årstadveien 19, 5009 Bergen, Norway.
Benef Microbes. 2018 Dec 7;9(6):865-874. doi: 10.3920/BM2018.0009. Epub 2018 Jul 25.
Probiotics can convert a dysbiotic bacterial environment into a healthy one. The aim of the present study was to assess the effect of daily intake of a probiotic milk drink on the composition of bacterial species in dental supra- and subgingival biofilms. Sixteen dental students were enrolled into this study with a crossover, within subject, design. The participants were asked to allow plaque accumulation by refraining from cleaning their molars during two separate periods, each lasting three weeks. Each period consisted of an initial professional dental cleaning procedure done at the university clinic, then a 3 week plaque accumulation period, followed by a return to the clinic for supra- and subgingival plaque sampling. The first period served as a control, and during the second plaque accumulation period the participants drank 200 ml probiotic milk beverage each day. The accumulated plaque removed at the end of the accumulation period was later tested against a panel of 20 oral bacterial species using the checkerboard method. Three weeks consumption of a probiotic beverage led to a significant reduction in 15 of 20 bacterial species present in supragingival plaque and a reduction in 4 of 20 bacterial species in subgingival plaque (all <0.05). This study showed a favorable effect of probiotics on periodontopathic bacteria in dental biofilms. The potential influence of this kind of probiotic in prevention or treatment of periodontal inflammation deserves further study.
益生菌可将失调的细菌环境转化为健康的环境。本研究旨在评估每日摄入益生菌牛奶饮料对牙上和牙下龈下生物膜中细菌种类组成的影响。本研究采用交叉、个体内设计，纳入了 16 名牙科学生。参与者被要求在两个不同的时间段内避免清洁磨牙，从而允许菌斑积累，每个时间段持续 3 周。每个时间段都包括在大学诊所进行的初始专业牙齿清洁程序，然后是 3 周的菌斑积累期，之后返回诊所进行龈上和龈下菌斑采样。第一阶段作为对照，在第二个菌斑积累阶段，参与者每天饮用 200 毫升益生菌牛奶饮料。在积累期结束时去除的积累菌斑随后使用 checkerboard 方法针对 20 种口腔细菌进行测试。益生菌饮料连续饮用 3 周可导致龈上菌斑中 20 种细菌中的 15 种显著减少，牙下菌斑中 20 种细菌中的 4 种减少（均<0.05）。本研究表明益生菌对牙周病细菌在牙生物膜中的生长具有有利影响。这种益生菌在预防或治疗牙周炎方面的潜在影响值得进一步研究。
