蒺藜苜蓿中的瞬时转录后基因沉默：病毒诱导的基因沉默（VIGS）

Transient Posttranscriptional Gene Silencing in Medicago truncatula: Virus-Induced Gene Silencing (VIGS).

作者信息

Serwatowska Joanna, Lund Ole Søgaard, Johansen Ida Elisabeth

机构信息

Unidad de Genómica Avanzada (LANGEBIO), CINVESTAV-IPN, Guanajuato, Mexico.

Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.

出版信息

Methods Mol Biol. 2018;1822:115-122. doi: 10.1007/978-1-4939-8633-0_8.

DOI:10.1007/978-1-4939-8633-0_8

PMID:30043300

Abstract

Successful application of virus-induced gene silencing for functional genomics requires a virus vector that can initiate a systemic infection of the host plant. Agroinoculation of the pea early browning virus vectors pCAPE1 and pCAPE2 can establish infection in several genotypes of Medicago truncatula and can reduce target gene RNA levels to an extent that allows investigation of gene function.

摘要

病毒诱导的基因沉默在功能基因组学中的成功应用需要一种能够引发宿主植物系统性感染的病毒载体。对豌豆早褐病毒载体pCAPE1和pCAPE2进行农杆菌接种，可以在多种基因型的蒺藜苜蓿中建立感染，并能将靶基因RNA水平降低到足以研究基因功能的程度。