Haratake J, Horie A, Nakashima A, Takeda S, Mori A
Arch Pathol Lab Med. 1986 Mar;110(3):192-4.
Minute hepatomas with prominent copper accumulations were resected in two women, aged 60 and 62 years, who had never suffered from jaundice. Mild elevation of serum alpha-fetoprotein level was found in both patients. One tumor was diagnosed by celiac angiography, and the other was determined by an ultrasonic echogram. Microscopically, these two tumors were relatively well-differentiated hepatocellular carcinoma, though having less-differentiated foci. Many cancer cells contained numerous copper granules stained by orcein, Victoria blue, and p-dimethylaminobenzylidene rhodanine. Ultrastructurally, cancer cells contained many secondary lysosomes with an electron-dense material. We concluded that the excessive copper in the cancer cells was aggregated lysosomal copper metallothionein, and that it might not be carcinogenic but stored by an altered metabolism of copper and copper-binding proteins with the neoplastic transformation.
在两名分别为60岁和62岁、从未患过黄疸的女性患者中，切除了伴有显著铜蓄积的微小肝癌。两名患者均发现血清甲胎蛋白水平轻度升高。其中一个肿瘤通过腹腔血管造影诊断，另一个通过超声心动图确定。显微镜下，这两个肿瘤为相对高分化的肝细胞癌，不过存在低分化灶。许多癌细胞含有大量被orcein、维多利亚蓝和对二甲氨基苄叉罗丹宁染色的铜颗粒。超微结构上，癌细胞含有许多带有电子致密物质的次级溶酶体。我们得出结论，癌细胞中过量的铜是聚集的溶酶体铜金属硫蛋白，它可能不具有致癌性，而是通过铜和铜结合蛋白代谢改变以及肿瘤转化而储存。
