Richardt G, Federolf G, Habermann E
Arch Toxicol. 1985 Sep;57(4):257-9. doi: 10.1007/BF00324788.
In the search for the mode of action of aluminum ions in dialysis encephalopathy, their interaction with calmodulin has been assessed, and compared with those of Ca2+, Pb2+, Mn2+, Hg2+ and Cd2+. The basal and calmodulin-dependent activity of phosphodiesterase was measured in the presence of the ions, and their binding to calmodulin was assessed by competition with 45Ca2+ in flow dialysis. Al3+, Mn2+, Hg2+, and Cd2+ cannot be regarded as exclusive calmodulin antagonists or agonists, but rather interact with the phosphodiesterase itself. Pb2+ however, mimicks Ca2+ in every system tested, and is active in the same concentration range as Ca2+. Our results indicate that the assumed role of Al3+ ions in dialysis encephalopathy or other neurological disturbances is not linked with calmodulin.
在探寻铝离子在透析性脑病中的作用方式时，已对其与钙调蛋白的相互作用进行了评估，并与钙离子、铅离子、锰离子、汞离子和镉离子的相互作用进行了比较。在有这些离子存在的情况下，测定了磷酸二酯酶的基础活性和钙调蛋白依赖性活性，并通过在流动透析中与45Ca2+竞争来评估它们与钙调蛋白的结合。铝离子、锰离子、汞离子和镉离子不能被视为唯一的钙调蛋白拮抗剂或激动剂，而是与磷酸二酯酶本身相互作用。然而，在每个测试系统中，铅离子都能模拟钙离子，并且在与钙离子相同的浓度范围内具有活性。我们的结果表明，铝离子在透析性脑病或其他神经功能紊乱中所假定的作用与钙调蛋白无关。
