通过气相色谱-质谱联用技术同时定量测定白三烯B4及其ω-氧化产物。

A simultaneous quantitation of leukotriene B4 and its omega-oxidized products by gas chromatography-mass spectrometry.

作者信息

Izumi T, Shimizu T, Kasama T, Seyama Y, Sumimoto H, Takeshige K, Minakami S, Wetterholm A, Rådmark O

出版信息

Biochem Biophys Res Commun. 1986 Jan 29;134(2):512-8. doi: 10.1016/s0006-291x(86)80450-8.

Abstract

We developed a highly sensitive and specific method for the simultaneous quantitation of leukotriene B4 (LTB4) and its omega-oxidized metabolites (20-hydroxy-LTB4 and 20-carboxy-LTB4) by mass fragmentography using deuterated compounds as internal standards. The ions produced by the cleavage of the C12-13 bond of the methyl ester dimethylisopropylsilyl ether derivatives of LTB4 and its metabolites were measured by selective ion monitorings. The detection limit of LTB4 was less than 10 pg and about 100-fold lower than that by high performance liquid chromatography. By using this method, the synthesis and further metabolism of LTB4 in human polymorphonuclear leukocytes were investigated.

摘要

我们开发了一种高度灵敏且特异的方法，以氘代化合物作为内标，通过质量碎片分析法同时定量白三烯B4（LTB4）及其ω-氧化代谢产物（20-羟基-LTB4和20-羧基-LTB4）。通过选择性离子监测来测定LTB4及其代谢产物的甲酯二甲基异丙基硅醚衍生物的C12-13键断裂所产生的离子。LTB4的检测限低于10 pg，比高效液相色谱法低约100倍。利用该方法，对白三烯B4在人多形核白细胞中的合成及进一步代谢进行了研究。