Dawson M, McGee C M, Brooks P M, Vine J H, Watson T R
Department of Rheumatology, Royal North Shore Hospital, St Leonards, NSW, Australia.
Biomed Environ Mass Spectrom. 1988 Sep;17(3):205-11. doi: 10.1002/bms.1200170309.
A procedure for the quantification of leukotriene B4 (LTB4) in synovial fluid has been developed using gas chromatography/tandem mass spectrometry based on selected reaction monitoring of the elimination of t-butyldimethylsilanol from the ions of m/z 431 and 438 in the negative ion chemical ionization mass spectra of the di-t-butyldimethylsilyl/pentafluorobenzyl derivatives of leukotriene B4 and the internal standard (2H8)leukotriene B4. The detection limit (approximately 10 pg ml-1) is sufficiently low to permit determination of LTB4 concentration in the synovial fluid of patients with various arthropathies. Single-stage mass spectrometry was found not to be selective enough to permit quantification of LTB4 in synovial fluid.
已开发出一种使用气相色谱/串联质谱法对滑液中白三烯B4（LTB4）进行定量的方法，该方法基于对白三烯B4及其内标（2H8）白三烯B4的二叔丁基二甲基硅烷基/五氟苄基衍生物负离子化学电离质谱中m/z 431和438离子消除叔丁基二甲基硅醇的选择反应监测。检测限（约10 pg ml-1）足够低，能够测定各种关节病患者滑液中的LTB4浓度。发现单级质谱的选择性不足以对滑液中的LTB4进行定量。
