一种干扰素类似物，[丙氨酸30,32,33]人干扰素α2，在牛细胞上作为人干扰素α2拮抗剂起作用。

An interferon analogue, [Ala 30,32,33]HuIFN-alpha 2, acting as a HuIFN-alpha 2 antagonist on bovine cells.

作者信息

Marcucci F, De Maeyer E

出版信息

Biochem Biophys Res Commun. 1986 Feb 13;134(3):1412-8. doi: 10.1016/0006-291x(86)90406-7.

Abstract

We have studied the biological and receptor binding properties of a human alpha 2-interferon (HuIFN-alpha 2) analogue, [Ala30,32,33] HuIFN-alpha 2, which is shown in the accompanying paper (1) to be biologically inactive on homologous cells. Here we demonstrate that this analogue is also devoid of biological activity on bovine MDBK cells. However, whereas the analogue did not inhibit the binding of radiolabeled HuIFN-alpha 2 to WISH cells, it did compete for binding to receptors on the bovine cells. This behavior suggested that [Ala30,32,33] HuIFN-alpha 2 could act as an antagonist of HuIFN-alpha 2 on bovine cells and indeed coaddition of the analogue and native HuIFN-alpha 2 to MDBK cells competitively inhibited both the antiviral and antiproliferative activity of HuIFN-alpha 2.

摘要

我们研究了一种人α2干扰素（HuIFN-α2）类似物，即[Ala30,32,33]HuIFN-α2的生物学和受体结合特性。随附论文（1）表明，该类似物对同源细胞无生物学活性。在此我们证明，这种类似物对牛MDBK细胞也没有生物学活性。然而，虽然该类似物不抑制放射性标记的HuIFN-α2与WISH细胞的结合，但它确实能竞争与牛细胞上受体的结合。这种行为表明，[Ala30,32,33]HuIFN-α2在牛细胞上可能作为HuIFN-α2的拮抗剂发挥作用，实际上，将该类似物与天然HuIFN-α2共同添加到MDBK细胞中，会竞争性抑制HuIFN-α2的抗病毒和抗增殖活性。