Fiola M R
Anal Quant Cytol Histol. 1985 Dec;7(4):299-304.
Quantitative methods for the routine diagnostic analysis of sural nerve biopsies were evaluated. Semiautomatic image analysis was used to compare three sampling methods and to test the accuracy and reproducibility of measurements of various elements in biopsy specimens from seven cases of paraproteinemic neuropathy. Systematic nonrandom sampling gave better results than did completely random sampling, as compared with systematic covering sampling. The accuracy of measurements was optimal at a final magnification of 3,200X. Comparison of single data of double measurements revealed a mean relative frequency of class change of 7.4%. Computation of regression showed good conformity, with r = 1.0. In conclusion, systematic nonrandom sampling at a final magnification of 3,200 X provided good analysis of the total population of structural elements with sufficient accuracy and reproducibility.
对腓肠神经活检常规诊断分析的定量方法进行了评估。采用半自动图像分析比较了三种采样方法，并测试了7例副蛋白血症性神经病活检标本中各种成分测量的准确性和可重复性。与系统覆盖采样相比，系统非随机采样比完全随机采样效果更好。在最终放大倍数为3200倍时测量的准确性最佳。双份测量的单个数据比较显示类别变化的平均相对频率为7.4%。回归计算显示出良好的一致性，r = 1.0。总之，在最终放大倍数为3200倍时进行系统非随机采样，能够以足够的准确性和可重复性对结构成分的总体进行良好分析。
