Gilmore T D, Temin H M
Cell. 1986 Mar 14;44(5):791-800. doi: 10.1016/0092-8674(86)90845-7.
Reticuloendotheliosis virus strain T (REV-T) is a highly oncogenic avian retrovirus that transforms early lymphoid cells in vivo and in vitro, but REV-T does not transform chicken embryo fibroblasts (CEF). Using antisera to p59v-rel, the v-rel oncogene product of REV-T, we show that p59v-rel is expressed at equal levels and is a phosphoprotein in REV-T infected spleen cells and CEF. Biochemical fractionation and immunofluorescence of REV-T infected nontransformed CEF show that p59v-rel is loosely associated with the nucleus. However, in REV-T transformed spleen cells p59v-rel is primarily a cytoplasmic protein. MSB-1 cells, a Marek's disease virus transformed T cell leukemic line, and E26 virus transformed myeloid cells show nuclear staining of p59v-rel when they are infected by REV-T. Our results indicate that there is a correlation between a cytoplasmic localization of p59v-rel and transformation by REV-T, and they suggest that p59v-rel cannot transform cells in which it assumes solely a nuclear location.
网状内皮组织增生症病毒T株（REV-T）是一种高度致癌的禽逆转录病毒，可在体内和体外转化早期淋巴细胞，但REV-T不能转化鸡胚成纤维细胞（CEF）。利用针对REV-T的v-rel癌基因产物p59v-rel的抗血清，我们发现p59v-rel在REV-T感染的脾细胞和CEF中以相等水平表达且是一种磷蛋白。对REV-T感染的未转化CEF进行生化分级分离和免疫荧光分析表明，p59v-rel与细胞核松散结合。然而，在REV-T转化的脾细胞中，p59v-rel主要是一种细胞质蛋白。MSB-1细胞（一种马立克氏病病毒转化的T细胞白血病系）和E26病毒转化的髓样细胞在被REV-T感染时显示p59v-rel的核染色。我们的结果表明，p59v-rel的细胞质定位与REV-T介导的转化之间存在相关性，并且提示p59v-rel不能转化那些它仅定位于细胞核的细胞。
