Pharmacology Division, Defence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Lucknow Road, Timarpur, Delhi 110 054, India.
Pharmacology Division, Defence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Lucknow Road, Timarpur, Delhi 110 054, India.
J Photochem Photobiol B. 2018 Sep;186:152-159. doi: 10.1016/j.jphotobiol.2018.07.008. Epub 2018 Jul 18.
Burn wound is a complex multi-factorial pathophysiology producing excruciating pain and psychological discomfort among patients, which imposes a major burden on the healthcare system. Multi-target therapy focuses on augmented healing by regulating different phases of tissue repair. Recently, photobiomodulation (PBM)-induced wound healing has achieved profound impetus as a non-invasive, drug-free biophysical therapeutic approach. On the other hand, medicinal honey known to possess antibacterial and immunomodulatory properties and is being used as an effective treatment option for infected wounds. The present study aimed to determine whether the combination of medicinal honey and PBM using superpulsed 904 nm laser treatment could additively accelerate full-thickness burn wound repair in rats. Animals were randomly allocated into 4 experimental groups: control (C), PBM superpulsed 904 nm laser treated (PBMT), honey treated (HT) and combined treatment (CT). The dual treatment exhibited an enhanced wound area contraction and hexosamine content as compared to the other groups. Histopathological analysis revealed increased cellular proliferation, extracellular matrix accumulation and decreased inflammation in the CT group. Further, the CT group demonstrated synergistically attenuated inflammation, pain and enhanced cell adhesion, migration as evidenced by significantly reduced protein expression of TNF-α, NF-κB, IL-1β, COX-2, substance-P receptor and up-regulation of fibronectin, respectively as compared with the other groups. Thus, the findings of present study signify that the combination of medicinal honey and PBMT accelerates the repair process of burn wounds. The study showed that therapeutic efficacy of 904 nm superpulsed laser-mediated PBM augments in the presence of medicinal honey by enhancing cellular proliferation and attenuation of inflammation and pain in burn wound healing.
烧伤创面是一种复杂的多因素病理生理学过程，会给患者带来剧烈的疼痛和心理不适，给医疗保健系统带来了重大负担。多靶点治疗侧重于通过调节组织修复的不同阶段来增强愈合。最近，光生物调节（PBM）诱导的伤口愈合作为一种非侵入性、无药物的生物物理治疗方法取得了深远的进展。另一方面，药用蜂蜜具有抗菌和免疫调节特性，被用作感染性伤口的有效治疗选择。本研究旨在确定药用蜂蜜和使用超脉冲 904nm 激光治疗的 PBM 的联合是否可以加速大鼠全层烧伤创面的修复。动物被随机分配到 4 个实验组：对照组（C）、PBM 超脉冲 904nm 激光治疗组（PBMT）、蜂蜜治疗组（HT）和联合治疗组（CT）。与其他组相比，双重治疗显示出更大的创面面积收缩和己糖胺含量增加。组织病理学分析显示 CT 组细胞增殖增加、细胞外基质积累增加、炎症减少。此外，与其他组相比，CT 组表现出协同抗炎、止痛作用，并增强细胞黏附、迁移，表现为 TNF-α、NF-κB、IL-1β、COX-2、P 物质受体的蛋白表达显著降低，纤连蛋白表达上调。因此，本研究的结果表明，药用蜂蜜和 PBMT 的联合加速了烧伤创面的修复过程。研究表明，904nm 超脉冲激光介导的 PBM 的治疗效果在药用蜂蜜存在的情况下增强，通过增强细胞增殖和减轻烧伤愈合过程中的炎症和疼痛。
