Suemaru S, Hashimoto K, Ota Z
Endocrinol Jpn. 1985 Oct;32(5):709-18. doi: 10.1507/endocrj1954.32.709.
Ether-laparotomy stress produced a rapid increase in rat hypothalamic CRF concentration, followed by a rapid reduction and subsequent increase. Cold-restraint stress significantly reduced hypothalamic CRF concentration at 15 min after stress onset. Serum ACTH and corticosterone levels were significantly elevated at 15 min after the onset of both stresses. The CRF responses in the medulla oblongata were not similar to the hypothalamic CRF responses. Norepinephrine concentration in the hypothalamus was reduced, whereas dopamine concentration in the hypothalamus and medulla oblongata was significantly increased. Epinephrine concentrations in these tissues did not show any significant change throughout the stress period. The observations lead to the following conclusions: hypothalamic CRF plays a major role in stimulating ACTH secretion under acute stress; the reduction in hypothalamic CRF is due to an excess release in the early phase of acute stress; hypothalamic CRF and medulla oblongata CRF are controlled by different mechanisms; norepinephrine in the hypothalamus may not be involved in stimulating hypothalamic CRF secretion in the early phase of acute stress; and catecholamines are regulated differently in the hypothalamus and medulla oblongata.
乙醚开腹应激使大鼠下丘脑促肾上腺皮质激素释放因子（CRF）浓度迅速升高，随后迅速降低，继而又升高。冷束缚应激在应激开始后15分钟时显著降低下丘脑CRF浓度。两种应激开始后15分钟时，血清促肾上腺皮质激素（ACTH）和皮质酮水平均显著升高。延髓中的CRF反应与下丘脑的CRF反应不同。下丘脑去甲肾上腺素浓度降低，而下丘脑和延髓中的多巴胺浓度显著升高。在整个应激期间，这些组织中的肾上腺素浓度未显示任何显著变化。这些观察结果得出以下结论：下丘脑CRF在急性应激下刺激ACTH分泌中起主要作用；下丘脑CRF的降低是由于急性应激早期的过度释放；下丘脑CRF和延髓CRF受不同机制控制；下丘脑去甲肾上腺素可能不参与急性应激早期刺激下丘脑CRF分泌；并且儿茶酚胺在下丘脑和延髓中的调节方式不同。
