Whole-genome sequencing identification of a multidrug-resistant Listeria monocytogenes serotype 1/2a isolated from fresh mixed sausage in southern Brazil.

A previous study performed by our group reported the characterization of a multidrug-resistant isolate of Listeria monocytogenes serotype 1/2a, named Lm16 isolate. This foodborne isolate is of particular interest because it is highly resistant to antimicrobials, disinfectants and heavy metals. In this study, we used the whole-genome shotgun method to sequence Lm16 isolate using the Illumina MiSeq platform. By assembling and analysis of the new genome, we were able to identify three efflux pumps (mepA, msrA, and norB) and mutations in the genes efTu and gyrA that confers resistance to antimicrobials. In conclusion, novel features in genome annotation regarding resistance genes in a L. monocytogenes foodborne isolate were identified.